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Letter
Nature Structural Biology  6, 565 - 568 (1999)
doi:10.1038/9347

The structure of a cypovirus and the functional organization of dsRNA viruses

Claire L. Hill1, 2, Timothy F. Booth1, 3, B. V. Venkataram Prasad4, Jonathan M. Grimes5, Peter P. C. Mertens6, Geoff C. Sutton5 & David I. Stuart5, 7

1  NERC Institute of Virology and Environmental Microbiology, Mansfield Road, Oxford, OX1 3SR, UK.

2  Present address: Second Floor Laboratories, Jefferiss wing, Imperial Medical School at St Mary's, Praed Street, London, W2 1NY, UK.

3  Present address: Canadian Food Inspection Agency, National Centre for Foreign Animal Disease, 1051 Arlington Street, Winnipeg, Manitoba, R3E 3M4, Canada.

4  Verna and Marrs McLean Department of Biochemistry, Baylor College of Medicine, 1 Baylor Plaza, Houston, Texas 77030, USA.

5  Laboratory of Molecular Biophysics, Rex Richards Building, South Parks Road, Oxford, OX1 3QU, UK.

6  Institute for Animal Health, Pirbright Laboratory, Ash Road, Pirbright, Woking, Surrey, GU24 0NF, UK.

7  The Oxford Centre for Molecular Sciences, New Chemistry Laboratory, Oxford, OX1 3QT, UK.

Correspondence should be addressed to David I. Stuart dave@biop.ox.ac.uk
Cytoplasmic polyhedrosis virus (CPV) is unique among the double-stranded RNA viruses of the family Reoviridae in having a single capsid layer. Analysis by cryo-electron microscopy allows comparison of the single shelled CPV and orthoreovirus with the high resolution crystal structure of the inner shell of the bluetongue virus (BTV) core. This suggests that the novel arrangement identified in BTV, of 120 protein subunits in a so-called 'T=2' organization, is a characteristic of the Reoviridae and allows us to delineate structural similarities and differences between two subgroups of the family — the turreted and the smooth-core viruses. This in turn suggests a coherent picture of the structural organization of many dsRNA viruses.

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Nature Structural & Molecular Biology
ISSN: 1545-9993
EISSN: 1545-9985
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