Abstract
Field tests of corn co-expressing two new delta-endotoxins from Bacillus thuringiensis (Bt) have demonstrated protection from root damage by western corn rootworm (Diabrotica virgifera virgifera LeConte). The level of protection exceeds that provided by chemical insecticides. In the bacterium, these proteins form crystals during the sporulation phase of the growth cycle, are encoded by a single operon, and have molecular masses of 14 kDa and 44 kDa. Corn rootworm larvae fed on corn roots expressing the proteins showed histopathological symptoms in the midgut epithelium.
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Acknowledgements
We thank Theodore Kahn for helpful discussions and contributions to preparing figures and reviewing this manuscript. We thank Leo Kim, Dave Grothaus, Larry Sernyk, and Roger Kemble for their organizational leadership and helpful discussions. We thank Steve Ritchie for his contribution in corn breeding, and Clara Alarcon and David Hondred for Southern analysis. The authors also wish to acknowledge the excellent technical work of Josh Russell.
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Moellenbeck, D., Peters, M., Bing, J. et al. Insecticidal proteins from Bacillus thuringiensis protect corn from corn rootworms. Nat Biotechnol 19, 668–672 (2001). https://doi.org/10.1038/90282
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DOI: https://doi.org/10.1038/90282
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