Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • News & Views
  • Published:

When FT-IR spectroscopy meets X-ray crystallography

Nature Structural Biology volume 8, pages 195–197 (2001)Cite this article

The first time-resolved FT-IR spectroscopic study of the photoactive yellow protein addresses the conflict between the X-ray structures of two early photocycle intermediates.

This is a preview of subscription content, access via your institution

Relevant articles

Open Access articles citing this article.

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Figure 1: Overall structure of the photoactive yellow protein.
Figure 2: Photocycle of the photoactive yellow protein.
Figure 3: The carbonyl stretching vibration of carboxylic amino acid side chains.
Figure 4: Sequence of structural changes in the active site of PYP (CPK color coding).

References

  1. Spudich, J.L. Mol. Microbiol. 28, 1051–1058 (1998).

    Article  CAS  Google Scholar 

  2. Brudler, R., Rammelsberg, R., Woo, T.W., Getzoff, E.D. & Gerwert, K. Nature Struct. Biol. 8 265–270 (2001).

    Article  CAS  Google Scholar 

  3. Perman, B. et al. Science 279, 1946–1950 (1998).

    Article  CAS  Google Scholar 

  4. Sprenger, W.W., Hoff, W.D., Armitage, J.P. & Hellingwerf, K.J. J Bacteriol 175, 3096–3104 (1993).

    Article  CAS  Google Scholar 

  5. Kort, R. et al. EMBO J. 15, 3209–3218 (1996).

    Article  CAS  Google Scholar 

  6. Jiang, Z. et al. Science 285, 406–409 (1999).

    Article  CAS  Google Scholar 

  7. Borgstahl, G.E., Williams, D.R. & Getzoff, E.D. Biochemistry 34, 6278–6287 (1995).

    Article  CAS  Google Scholar 

  8. Düx, P. et al. Biochemistry 37, 12689–12699 (1998).

    Article  Google Scholar 

  9. Ng, K., Getzoff, E.D. & Moffat, K. Biochemistry 34, 879–890 (1995).

    Article  CAS  Google Scholar 

  10. Mäntele, W. Trends Biochem. Sci. 18, 197–202 (1993).

    Article  Google Scholar 

  11. Uhmann, W., Becker, A., Taran, C. & Siebert, F. Appl. Spectrosc. 45, 390–397 (1991).

    Article  CAS  Google Scholar 

  12. Heberle, J. Biochim. Biophys. Acta. 1458, 135–147 (2000).

    Article  CAS  Google Scholar 

  13. Moffat, K. Methods Enzymol. 277, 433–447 (1997).

    Article  CAS  Google Scholar 

  14. Ujj, L. et al. Biophys. J. 75, 406–412 (1998).

    Article  CAS  Google Scholar 

  15. Genick, U.K., Soltis, S.M., Kuhn, P., Canestrelli, I.L. & Getzoff, E.D. Nature 392, 206–209 (1998).

    Article  CAS  Google Scholar 

  16. Devanathan, S. et al. Biophys. J. 77, 1017–1023 (1999).

    Article  CAS  Google Scholar 

  17. Imamoto, Y., Kataoka, M. & Tokunaga, F. Biochemistry 35, 14047–14053 (1996).

    Article  CAS  Google Scholar 

  18. Masciangioli, T., Devanathan, S., Cusanovich, M.A., Tollin, G. & El-Sayed, M.A. Photochem. Photobiol. 72, 639–644 (2000).

    Article  CAS  Google Scholar 

  19. Meyer, T.E., Tollin, G., Hazzard, J.H. & Cusanovich, M.A. Biophys. J. 56, 559–564 (1989).

    Article  CAS  Google Scholar 

  20. van Brederode, M.E., Hoff, W.D., van Stokkum, I.H., Groot, M.L. & Hellingwerf, K.J. Biophys. J. 71, 365–380 (1996).

    Article  CAS  Google Scholar 

  21. Hoff, W.D. et al. Biochemistry 38, 1009–1017 (1999).

    Article  CAS  Google Scholar 

  22. Salamon, Z., Meyer, T.E. & Tollin, G. Biophys. J. 68, 648–654 (1995).

    Article  CAS  Google Scholar 

  23. Meyer, T.E., Cusanovich, M.A. & Tollin, G. Arch. Biochem. Biophys. 306, 515–517 (1993).

    Article  CAS  Google Scholar 

  24. Hendriks, J., Hoff, W.D., Crielaard, W. & Hellingwerf, K.J. J. Biol. Chem. 274, 17655–17660 (1999).

    Article  CAS  Google Scholar 

  25. Craven, C.J. et al. Biochemistry 39, 14392–14399 (2000).

    Article  CAS  Google Scholar 

  26. Rubinstenn, G. et al. Nature Struct. Biol. 5, 568–570 (1998).

    Article  CAS  Google Scholar 

  27. Genick, U.K. et al. Science 275, 1471–1475 (1997).

    Article  CAS  Google Scholar 

  28. Xie, A. et al. Biochemistry 40 (2001).

  29. Hoff, W.D. et al. Biophys. J. 67, 1691–1705 (1994).

    Article  CAS  Google Scholar 

Download references

Acknowledgements

We thank G. Büldt and J. Labahn for helpful discussions on the interpretation of X-ray diffraction data.

Author information

Author notes

  1. Joachim Heberle and Thomas Gensch: J.H. is in Structural Biology and T.G. is in Cellular Signal Processing.

Authors and Affiliations

  1. the Forschungszentrum Jülich, Jülich, 52425, Germany

    Joachim Heberle & Thomas Gensch

Authors
  1. Joachim Heberle
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Thomas Gensch
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Joachim Heberle.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Heberle, J., Gensch, T. When FT-IR spectroscopy meets X-ray crystallography. Nat Struct Mol Biol 8, 195–197 (2001). https://doi.org/10.1038/84909

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1038/84909

This article is cited by

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing