Abstract
MUTAGENESIS induced by DNA damage in Saccharomyces cerevisiae requires the products of the REV1, REV3 and REV7 genes1. The Rev3 and Rev7 proteins are subunits of DNA polymerase-zeta2 (Pol-ζ), an enzyme whose sole function appears to be translesion synthesis3. Rev1 protein has weak homology with UmuC protein4, which facilitates translesion synthesis in Escherichia coli by an unknown mechanism. We show here that Revl protein has a deoxycytidyl transferase activity which transfers a dCMP residue from dCTP to the 3′ end of a DNA primer in a template-dependent reaction. Efficient transfer occurred opposite a template abasic site, but ∼20% transfer also occurred opposite a template guanine and ∼10% opposite adenine or uracil; ≤1% was seen opposite thymine or cytosine. Insertion of cytosine opposite an abasic site produced a terminus that was extended efficiently by Pol-ζ, but not by yeast Pol-α.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Lawrence, C. W. BioEssays 16, 253–258 (1994).
Nelson, J. R., Lawrence, C. W. & Hinkle, D. C. Science 272, 1646–1649 (1996).
Morrison, A. et al. J. Bact. 171, 5659–5667 (1989).
Larimer, F. W., Perry, J. R. & Hardigree, A. A. J. Bact 171, 230–237 (1989).
Gibbs, P. E. M. & Lawrence, C. W. J. molec. Biol. 251, 229–236 (1995).
Lindahl, T. & Nyberg, B. Biochemistry 11, 3610–3618 (1972).
Armstrong, J. D. & Kunz, B. A. Proc. natn. Acad. Sci. U.S.A. 87, 9005–9009 (1990).
Gibbs, P. E. M., Kilbey, B. J., Banerjee, S. K. & Lawrence, C. W. J. Bact. 175, 2607–2612 (1993).
Gibbs, P. E. M., Borden, A. & Lawrence, C. W. Nucleic Acids Res. 23, 1919–1922 (1995).
Ratliff, R. L. in The Enzymes Vol XIV A (ed. Boyer, P. D.) 105–118 (Academic, New York, 1981).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Nelson, J., Lawrence, C. & Hinkle, D. Deoxycytidyl transferase activity of yeast REV1 protein. Nature 382, 729–731 (1996). https://doi.org/10.1038/382729a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/382729a0
This article is cited by
-
Mechanism of nucleotide discrimination by the translesion synthesis polymerase Rev1
Nature Communications (2022)
-
Mechanisms of APOBEC3 mutagenesis in human cancer cells
Nature (2022)
-
Genetic and physical interactions between Polη and Rev1 in response to UV-induced DNA damage in mammalian cells
Scientific Reports (2021)
-
Developmental delay with hypotrophy associated with homozygous functionally relevant REV3L variant
Journal of Molecular Medicine (2021)
-
The in vivo role of Rev1 in mutagenesis and carcinogenesis
Genes and Environment (2020)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.
