Abstract
DURING mammalian development, many cells are programmed to die1,2 most mediated by apoptosis3. The Fas antigen4 coded by the structural gene for mouse lymphoproliferation mutation (lpr)5,6, is a cell surface protein belonging to the tumour necrosis factor/nerve growth factor receptor family7,8, and mediates apoptosis7. The Fas antigen messenger RNA is expressed in the thymus, liver, heart, lung and ovary8. We prepared a monoclonal antibody against mouse Fas antigen, which immunoprecipitated the antigen (Mr 45K) and had cytolytic activity against cell lines expressing mouse Fas antigen. We report here that staining of mouse thymocytes with the antibody indicated that thymocytes from the wild-type and lprcg mice expressed the Fas antigen, whereas little expression of the Fas antigen was found in lpr mice. Intraperitoneal administration of the anti-Fas antibody into mice rapidly killed the wild-type mice but neither lpr nor lprcg mice. Biochemical, histological and electron microscope analyses indicated severe damage of the liver by apoptosis. These findings suggest that the Fas antigen is important in programmed cell death in the liver, and may be involved in fulminant hepatitis in some cases.
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References
Raff, M. C. Nature 356, 397–400 (1992).
Ellis, R. E., Yuan J. & Horvitz, H. R. A. Rev. Cell Biol. 7, 663–698 (1991).
Wyllie, A. H., Kerr, J. F. R. & Currie, A. R. Int. Rev. Cytol. 68, 251–306 (1980).
Yonehara, S., Ishii, A. & Yonehara, M. J. exp. Med. 169, 1747–1756 (1989).
Watanabe-Fukunaga, R., Brannan, C. I., Copeland, N. G., Jenkins, N. A. & Nagata, S. Nature 356, 314–317 (1992).
Adachi, M., Watanabe-Fukunaga, R. & Nagata, S. Proc. natn. Acad. Sci. U.S.A. 90, 1756–1760 (1993).
Itoh, N. et al. Cell 66, 233–243 (1991).
Watanabe-Fukunaga, R. et al. J. Immun. 148, 1274–1279 (1992).
Meier, T., Arni, S., Malarkannan, S., Poincelet, M. & Hoessli, D. Analyt. Blochem. 204, 220–226 (1992).
Kimura, M. et al. Immunology 76, 498–504 (1992).
Trauth, B. C. et al. Science 245, 301–305 (1989).
Oehm, A. et al. J. biol. Chem. 267, 10709–10715 (1992).
Lehmann, V., Freudenberg, M. A. & Galanos, C. J. exp. Med. 165, 657–663 (1993).
Eskandari, M. K. et al. J. Immun. 148, 2724–2730 (1992).
Watson, J., Kelly, K., Largen, M. & Taylor, B. A. J. Immun. 120, 422–424 (1978).
Waage, A. & Espevik, T. J. exp. Med. 167, 1987–1992 (1988).
Tracey, K. J. et al. Science 234, 470–474 (1986).
Benedetti, A., Jezaquel, A. M. & Orlandi, F. J. Hepat. 7, 319–324 (1988).
Potten, C. S. Cancer Metastasis Rev. 11, 95–103 (1992).
Chisari, F. V. Molec. Gen. Med. 2, 67–103 (1992).
Rouvier, E., Luciani, M.-F. & Golstein, P. J. exp. Med. 177, 195–200 (1993).
Falk, M. H. Blood 79, 3300–3306 (1992).
Debatin, K.-M., Goldmann, C. K., Bamford, R., Waldman, T. A. & Krammer, P. H. Lancet 335, 497–500 (1990).
Kobayashi, N. et al. Proc. natn. Acad. Sci. U.S.A. 87, 9620–9624 (1990).
Sanchez-Madrid, F., Szklut, P. & Springer, T. A. J. Immun. 130, 309–312 (1983).
Hirata, Y. et al. J. Immun. 143, 2900–2906 (1989).
Cligan, J. E., Kruisbeek, A. M., Hargulies, D. H., Shevach, E. M. & Strober, W. Current Protocols in Immunology (Wiley Interscience, New York, 1991).
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Ogasawara, J., Watanabe-Fukunaga, R., Adachi, M. et al. Lethal effect of the anti-Fas antibody in mice. Nature 364, 806–809 (1993). https://doi.org/10.1038/364806a0
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DOI: https://doi.org/10.1038/364806a0
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