Abstract
ELONGATION factor-lα (EF-lα), an essential component of the eukaryotic translational apparatus, is a GTP-binding protein that catalyses the binding of aminoacyl-transfer RNAs to the ribosome1–3. Expression of the EF-lα gene decreases towards the end of the lifespans of mouse and human fibroblasts4,5, but forced expression of EF-lα prolongs the lifespan of Drosophila melanogaster6. Eukaryotic initiation factor-4E, another component of the translational machinery, is mitogenic or oncogenic when constitutively expressed in some mammalian cells7–9. Thus, components of the protein synthesis apparatus seem to be involved in the control of cell proliferation. Using expression cloning, we have isolated a complementary DNA clone from a BALB/c 3T3 mouse fibroblast variant, A31-I-13 (ref. 10), which specifies a factor determining the susceptibility of BALB/c 3T3 to chemically and physically induced transformation. Here we report that the factor is EF-lα and that its constitutive expression causes BALB/c 3T3 A31-I-1 (ref. 10), C3H10T1/2 (ref. 11) and Syrian hamster SHOK12 fibroblasts to become highly susceptible to transforma-tion induced by 3-methylcholanthrene and ultraviolet light. EF-lα messenger RNA is also constitutively expressed in a quiescent culture of the highly susceptible variant A31-1-13. We conclude that the removal of regulation of the expression of these com-ponents of the translational machinery may predispose cells to become more susceptible to malignant transformation.
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Tatsuka, M., Mitsui, H., Wada, M. et al. Elongation factor-1α gene determines susceptibility to transformation. Nature 359, 333–336 (1992). https://doi.org/10.1038/359333a0
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DOI: https://doi.org/10.1038/359333a0
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