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Oceanic 18S rDNA sequences from picoplankton reveal unsuspected eukaryotic diversity

Nature volume 409, pages 607–610 (2001)Cite this article

Abstract

Picoplankton—cells with a diameter of less than 3 µm—are the dominant contributors to both primary production and biomass in open oceanic regions1,2. However, compared with the prokaryotes3, the eukaryotic component of picoplankton is still poorly known. Recent discoveries of new eukaryotic algal taxa based on picoplankton cultures4,5 suggest the existence of many undiscovered taxa. Conventional approaches based on phenotypic criteria have limitations in depicting picoplankton composition due to their tiny size and lack of distinctive taxonomic characters6. Here we analyse, using an approach that has been very successful for prokaryotes7 but has so far seldom been applied to eukaryotes8, 35 full sequences of the small-subunit (18S) ribosomal RNA gene derived from a picoplanktonic assemblage collected at a depth of 75 m in the equatorial Pacific Ocean, and show that there is a high diversity of picoeukaryotes. Most of the sequences were previously unknown but could still be assigned to important marine phyla including prasinophytes, haptophytes, dinoflagellates, stramenopiles, choanoflagellates and acantharians. We also found a novel lineage, closely related to dinoflagellates and not previously described.

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Figure 1: Phylogenetic tree based on nearly complete sequences of the 18S rRNA gene.

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Acknowledgements

S.Y.M.-v.d.S. was supported by a ‘Poste Rouge’ from CNRS. This work was also funded by the European Framework Programme 5 PICODIV and the French programmes ACC-SV7 Biosystematics network and PROOF PROSOPE.

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Author notes

  1. Seung Yeo Moon-van der Staay

    Present address: Department of Evolutionary Microbiology, University of Nijmegen, NL-6525ED, The Netherlands

Authors and Affiliations

  1. Station Biologique, UPR 9042 Centre National de la Recherche Scientifique et Université Pierre et Marie Curie, BP 74, Roscoff Cedex, 29682, France

    Seung Yeo Moon-van der Staay & Daniel Vaulot

  2. Departement Biochemie, Universiteit Antwerpen (UIA), Universiteitsplein 1, Antwerpen, B-2610, Belgium

    Rupert De Wachter & Daniel Vaulot

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  1. Seung Yeo Moon-van der Staay
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  3. Daniel Vaulot
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Correspondence to Daniel Vaulot.

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Moon-van der Staay, S., De Wachter, R. & Vaulot, D. Oceanic 18S rDNA sequences from picoplankton reveal unsuspected eukaryotic diversity. Nature 409, 607–610 (2001). https://doi.org/10.1038/35054541

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