Nature 342, 243 - 247 (16 November 1989); doi:10.1038/342243a0

Scanning from an independently specified branch point defines the 3' splice site of mammalian introns

Christopher W. J. Smith, Elena B. Porro, James G. Patton & Bernardo Nadal-Ginard

Laboratory of Molecular and Cellular Cardiology, Howard Hughes Medical Institute, Department of Cardiology, Children's Hospital, and Departments of Pediatrics and Cellular and Molecular Physiology, Harvard Medical School, Boston, Massachusetts 02115, USA

During pre-messenger RNA splicing the lariat branch point in mammalian introns is specified independently of the 3' splice site by the sequence surrounding the branch point and by an adjacent downstream polypyrimidine tract. The 3' splice site is dispensable for spliceosome assembly and cleavage at the 5' splice site, and is itself determined by a scanning process that recognizes the first AG located 3' of the branch point/poly-pyrimidine tract, irrespective of distance or sequence environment.

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