Nature 336, 487 - 489 (01 December 1988); doi:10.1038/336487a0

Laminin receptor on platelets is the integrin VLA-6

Arnoud Sonnenberg^*, Piet W. Modderman^* & Frans Hogervorst^†

^*Central Laboratory of the Netherlands Red Cross Blood Transfusion Service and Laboratory for Experimental and Clinical Immunology, University of Amsterdam, The Netherlands
^†The Netherlands Cancer Institute, Amsterdam, The Netherlands

Adhesion of platelets to the subendothelial matrix of an injured vessel wall is an essential step in triggering the formation of a haemostatic plug. Fibronectin, collagen and laminin are three major components of the subendothelial matrix which support platelet adhesion¹. Receptors for fibronectin and collagen have been identified on platelets and are included in the integrin family²⁻¹¹. Here we report that adhesion of platelets to laminin is inhibited by a rat monoclonal antibody against the integrin family member, VLA-6. This antibody does not affect platelet adhesion to fibrinogen, fibronectin or to type I and III collagen. Binding to laminin does not require platelet activation and is not inhibited by fibronectin and laminin cell-attachment peptides. Platelet adhesion to laminin is supported by Mn²⁺, Co²⁺and Mg²⁺, but not by Ca²⁺, Zn²⁺ and Cu²⁺. This cation preference is distinct from that characteristic for other platelet-adhesive glycoproteins^8,12,13.

References

1.	III, C. R., Engvall, E. & Ruoslahti, E. J. Cell Biol. 99, 2138−2145 (1984).
2.	Gardner, J. M. & Hynes, R. O. Cell 42, 439−448 (1985). | Article | PubMed | ISI | ChemPort |
3.	Plow, E. F., McEver, R. P., Coller, B. S., Woods, V. L. Jr & Marguerie, G. A. Blood 66, 724−727 (1985). | PubMed | ChemPort |
4.	Pytela, R., Pierschbacher, M. D., Ginsberg, M. H., Plow, E. F. & Ruoslahti, E. Science 231, 1559−1562 (1986). | PubMed | ISI | ChemPort |
5.	Giancotti, F. P. et al. Blood 69, 1535−1538 (1987). | PubMed | ChemPort |
6.	Piotrowicz, R. S., Orchekowski, R. P., Nugent, D. J., Yamada, K. Y. & Kunicki, T. J. J. Cell Biol 106, 1359−1364 (1988). | Article | PubMed | ChemPort |
7.	Nieuwenhuis, H. K., Akkerman, J. W. N., Houdijk, W. P. M. & Sixma, J. J. Nature 318, 470−472 (1985). | Article | PubMed | ISI | ChemPort |
8.	Santoro, S. A. Cell 46, 913−920 (1986). | Article | PubMed | ISI | ChemPort |
9.	Santoro, S. A., Rajpara, S. M., Staatz, W. D. & Woods, V. J. Jr Biochem. biophys. Res. Commun. 153, 217−223 (1988). | Article | PubMed | ISI | ChemPort |
10.	Kunicki, T. J. et al. J. biol. Chem. 263, 4516−4519 (1988). | PubMed | ChemPort |
11.	Hemler, M. E., Crouse, C., Takada, Y. & Sonnenberg, A. J. biol. Chem. 263, 7660−7665 (1988). | PubMed | ChemPort |
12.	Bennett, J. S. & Vilaire, G. J. clin. Invest. 64, 1393−1401 (1979). | PubMed | ISI | ChemPort |
13.	Marguerie, G. A., Edgington, T. S. & Plow, E. F. J. biol. Chem. 255, 154−161 (1980). | PubMed | ChemPort |
14.	Hynes, R. O. Cell 48, 549−554 (1987). | Article | PubMed | ISI | ChemPort |
15.	Ruoslahti, E. & Pierschbacher, M. D. Science 238, 491−497 (1987). | PubMed | ISI | ChemPort |
16.	Jennings, L. & Phillips, D. R. J. biol. Chem. 257, 10458−10463 (1982). | PubMed | ChemPort |
17.	Pischel, K. D., Bluestein, H. G. & Woods, V. L. J. clin. Invest. 81, 505−513 (1987).
18.	Pytela, R., Pierschbacher, M. D. & Ruoslahti, E. Cell 40, 191−198 (1985). | Article | PubMed | ISI | ChemPort |
19.	Takada, Y., Huang, C. & Hemler, M. E. Nature 326, 607−609 (1987). | Article | PubMed | ChemPort |
20.	Sonnenberg, A., Janssen, H., Hogervorst, F., Calafat, J. & Hilgers, J. J. biol. Chem. 262, 10376−10383 (1987). | PubMed | ISI | ChemPort |
21.	Rao, N. C., Barsky, S. H., Terranova, V. P. & Liotta, L. A. Biochem. biophys. Res. Commun. 111, 804−808 (1983). | Article | PubMed | ISI | ChemPort |
22.	Malinoff, H. L. & Wicha, M. S. J. Cell Biol. 96, 1475−1479 (1983). | Article | PubMed | ChemPort |
23.	Lesot, H., Kühl, U. & von der Mark, K. EMBO J. 2, 861−865 (1983). | ISI | ChemPort |
24.	Wayner, E. A. & Carter, W. G. J. Cell. Biol. 105, 1873−1884 (1987). | Article | PubMed | ChemPort |
25.	Graf, J. et al. Cell 48, 989−996 (1987). | Article | PubMed | ISI | ChemPort |
26.	Modderman, P. W., Huisman, H. G., van Mourik, J. A. & von dem Borne, A. E. G. Kr. Thromb. Haemostas 60, 68−74 (1988). | ChemPort |
27.	Giltay, J. C., Brinkman, H. J. M., Modderman, P. W., von dem Borne, A. E. G. Kr. & van Mourik, J. A. Blood (submitted).
28.	Cheresh, D. A. et al. J. Cell Biol. 105, 1163−1173 (1987). | Article | PubMed | ChemPort |
29.	Edgar, D., Timpl, R. & Thoenen, H. EMBO J. 3, 1463−1468 (1984). | PubMed | ISI | ChemPort |
30.	Engvall, E. et al. J. Cell Biol. 103, 2457−2465 (1986). | Article | PubMed | ISI | ChemPort |
31.	Legrand, C., Dubernard, V. & Nurden, A. T. Biochim. biophys. Acta 812, 802−810 (1985). | PubMed | ChemPort |