Nature 335, 694 - 699 (20 October 1988); doi:10.1038/335694a0

NMR evidence for an early framework intermediate on the folding pathway of ribonuclease A

Jayant B. Udgaonkar & Robert L. Baldwin

Department of Biochemistry, Stanford University School of Medicine, Stanford, California 94305, USA

The presence of an early intermediate on the folding pathway of ribonuclease A has been demonstrated by a study of the exchange reaction between the backbone amide protons in the folding protein and solvent protons using rapid mixing techniques. A structural analysis of the intermediate by two-dimensional ¹H-NMR is consistent with the framework model of protein folding in which stable secondary structure first forms the framework necessary for the subsequent formation of the complete tertiary structure.

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