Abstract
Complementary DNA cloning of the transcripts of the Drosophila clock gene period reveals three distinct transcripts. These result from unusual splicing pathways, one involving a CG 3′ splice site and one resulting in the use of two different reading frames in one exon, and they predict three separate proteins. Two of the cloned cDNAs can restore clock function to mutant arrhythmic flies.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Turek, F. W. A. Rev. Physiol. 47, 49–64 (1985).
Konopka, R. J. & Benzer, S. Proc. natn. Acad. Sci. U.S.A. 68, 2112–2116 (1971).
Hall, J. C. Devl Genet. 4, 355–378 (1984).
Kyriacou, C. P. & Hall, J. C. Proc. natn. Acad. Sci. U.S.A. 77, 6729–6733 (1980).
Bargiello, T. A. & Young, M. W. Proc. natn. Acad. Sci. U.S.A. 81, 2142–2146 (1984).
Reddy, P. et al. Cell 38, 701–710 (1984).
Zehring, W. A. et al. Cell 39, 369–376 (1984).
Bargiello, T. A., Jackson, F. R. & Young, M. W. Nature 312, 752–754 (1984).
James, A. A., Ewer, J., Reddy, P., Hall, J. C. & Rosbash, M. EMBO J. 5, 2313–2320 (1986).
Jackson, F. R., Bargiello, T. A., Yun, S. H. & Young, M. W. Nature 320, 185–188 (1986).
Reddy, P., Jacquier, A. C., Abovich, N., Petersen, G. & Rosbash, M. Cell 46, 53–61 (1986).
Bourdon, M. A., Oldberg, A., Pierschbacher, M. & Ruoslahti, E. Proc. natn. Acad. Sci. U.S.A. 82, 1321–1325 (1985).
Padgett, R. A., Grabowski, P. J., Konarska, M. M., Seiler, S. & Sharp, P. A. A. Rev. Biochem. 55, 1119–1150 (1986).
Sanger, F. et al. Nature 265, 687–695 (1977).
Bos, J. L. et al. Cell 27, 121–131 (1981).
Spindel, E. R., Zilberberg, M. D., Habener, J. F. & Chin, W. W. Proc. natn. Acad. Sci. U.S.A. 83, 19–23 (1986).
King, C. R. & Piatigorsky, J. Cell 32, 707–712 (1983).
Dodgson, J. B. & Engel, J. D. J. biol. Chem. 258, 4623–4629 (1983).
Dush, M. K., Sikela, J. M., Khan, S. A., Tischfield, J. A. & Stambrook, P. J. Proc. natn. Acad. Sci. U.S.A. 82, 2731–2735 (1985).
Erbil, C. & Niessing, J. EMBO J. 2, 1339–1343 (1983).
Levanon, D. et al. EMBO J. 4, 77–84 (1985).
Gilbert, W. Nature 271, 501 (1978).
Treisman, R., Orkin, S. H. & Maniatis, T. Nature 302, 591–596 (1983).
Davis, R. L. & Davidson, N. Molec. cell. Biol. 6, 1464–1470 (1986).
Yu, Q. et al. Proc. natn. Acad. Sci. U.S.A. (in the press).
Weitzel, G. & Rensing, L. J. comp. Physiol. 143, 229–235 (1981).
Kyriacou, C. P. & Hall, J. C. Science 232, 474–497 (1986).
Konopka, R. J., Wells, S. & Lee, T. Molec. gen. Genet. 190, 284–288 (1983).
Anderson, J. M. & Fambrough, D. M. J. Cell Biol. 97, 1396–1411 (1983).
Matthew, W. D. & Patterson, P. H. Cold Spring Harb. Symp. quant. Biol. 48, 625–631 (1983).
Carey, D. J. & Todd, M. S. J. biol. Chem. 261, 7518–7525 (1986).
Cole, G. J., Loewy, A. & Glaser, L. Nature 320, 445–447 (1986).
Jaffe, E. & Laird, C. Trends Genet. 2, 316–321 (1986).
Smith, R. F. & Konopka, R. J. Molec gen. Genet. 185, 30–36 (1982).
Hamblen et al. J. Neurogenet. 3, 249–291 (1986).
Barnett, J., Pachl, C., Gergen, J. P. & Wensink, P. Cell 21, 729–738 (1980).
Vincent, A., O'Connell, P., Gray, M. R. & Rosbash, M. EMBO J. 3, 1003–1013 (1984).
Aviv, H. & Leder, P. Proc. natn. Acad. Sci. U.S.A. 69, 1408–1412 (1972).
Feinberg, A. P. & Vogelstein, B. Analyt. Biochem. 132, 6–13 (1983).
Gubler, V. & Hoffman, B. J. Gene 25, 263–269 (1983).
Ben-Neriah, Y., Bernards, A., Paskind, M., Daley, G. Q. & Baltimore, D. Cell 44, 577–586 (1986).
Young, R. A. & Davis, R. W. Proc. natn. Acad. Sci. U.S.A. 80, 1194–1198 (1983).
Maxam, A. M. & Gilbert, W. Proc. natn. Acad. Sci. U.S.A. 74, 560–564 (1977).
Kozak, M. Cell 44, 283–292 (1986).
Mount, S. M. Nucleic Acids Res. 10, 459–472 (1982).
Rubin, G. M. Trends Neurosci. 8, 231–233 (1985).
Henikoff, S. Gene 28, 351–359 (1984).
Sanger, F., Nicklen, S. & Coulson, A. Proc. natn. Acad. Sci. U.S.A. 74, 5463–5467 (1977).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Citri, Y., Colot, H., Jacquier, A. et al. A family of unusually spliced biologically active transcripts encoded by a Drosophila clock gene. Nature 326, 42–47 (1987). https://doi.org/10.1038/326042a0
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1038/326042a0
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.
