Abstract
DNAs from four out of five patients with acute myeloid leukaemia (AML) tested by an in vivo selection assay in nude mice using transfected mouse NIH 3T3 cells were found to contain an activated N-ras oncogene. Using a set of synthetic oligonucleotide probes, we have detected a mutation at codon 13 in all four genes. The same codon is mutated in an additional AML DNA that is positive in the focus-formation assay on 3T3 cells. DNA from the peripheral blood of one patient in remission does not contain a codon 13 mutation.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Rent or buy this article
Prices vary by article type
from$1.95
to$39.95
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Land, H., Parada, L. F. & Weinberg, R. A. Science 222, 771–778 (1983).
Cooper, G. M. Science 218, 801–806 (1982).
Tabin, C. J. et al. Nature 300, 143–149 (1982).
Reddy, E. P., Reynolds, R. K., Santos, E. & Barbacid, M. Nature 300, 149–152 (1982).
Taparowsky, E. et al. Nature 300, 762–765 (1982).
Yuasa, Y. et al. Nature 303, 775–779 (1983).
Shimizu, K. et al. Nature 304, 497–500 (1983).
Capon, D. J. et al. Nature 304, 507–513 (1983).
Santos, E. et al. Science 223, 661–664 (1984).
Nakano, H. et al. Proc. natn. Acad. Sci. U.S.A. 81, 71–75 (1984).
Tainsky, M. A., Cooper, C. S., Giovanella, B. C. & Vande Woude, G. F. Science 223, 643–645 (1984).
Taparowsky, E., Shimizu, K., Goldfarb, M. & Wigler, M. Cell 34, 581–586 (1983).
Brown, R., Marshall, C. J., Pennie, S. G. & Hall, A. EMBO J. 3, 1321–1326 (1984).
Kraus, M. H., Yuasa, Y. & Aaronson, S. A. Proc. natn. Acad. Sci. U.S.A. 81, 5384–5388 (1984).
Yuasa, Y. et al. Proc. natn. Acad. Sci. U.S.A. 84, 3670–3674 (1984).
Fujita, J. et al. Nature 309, 464–466 (1984).
Bos, J. L. et al. Nucleic Acids Res. 12, 9155–9163 (1984).
Fasano, O. et al. Proc. natn. Acad. Sci. U.S.A. 81, 4008–4012 (1984).
Bennett, J. M. et al. Br. J. Haemat. 33, 451–458 (1976).
Blair, D. G., Cooper, C. S., Oskarsson, M. K., Eader, L. A. & Vande Woude, G. F. Science 218, 1122–1124 (1982).
Seeburg, P. H., Colby, W. W., Capon, D. J., Goeddel, D. V. & Levinson, A. D. Nature 312, 71–74 (1984).
Southern, P. J. & Berg, P. J. molec. appl. Genet. l, 327–341 (1982).
Fasano, O., Birnbaum, D., Edlund, L., Fogh, J. & Wigler, M. Molec. cell. Biol. 4, 1695–1705 (1984).
Hall, A., Marshall, C. J., Spurr, N. K. & Weiss, R. A. Nature 303, 396–400 (1983).
Conner, B. J. et al. Proc. natn. Acad. Sci. U.S.A. 80, 278–282 (1983).
Maxam, A. & Gilbert, W. Proc. natn. Acad. Sci. U.S.A. 74, 560–564 (1977).
Eva, A., Tronick, S. R., Gol, R. A., Pierce, J. H. & Aaronson, S. A. Proc. natn. Acad. Sci. U.S.A. 80, 4926–4930 (1983).
Murray, M. J. et al. Cell 33, 749–757 (1983).
Gambke, C., Signer, E. & Moroni, C. Nature 307, 476–478 (1984).
Souyri, M. & Fleissner, E. Proc. natn. Acad. Sci. U.S.A. 80, 6676–6679 (1983).
Sukumar, S., Notario, V., Martin-Zanca, D. & Barbacid, M. Nature 306, 658–661 (1983).
Balmain, A. & Pragnell, I. B. Nature 303, 72–74 (1983).
Guerrero, I., Calzada, P., Mayer, A. & Pellicer, A. Proc. natn. Acad. Sci. U.S.A. 81, 202–205 (1984).
Moroni, C., Hall, A. & Gambke, C. Proc. natn. Acad. Sci. U.S.A. (in the press).
Yunis, J. J. Science 221, 227–236 (1984).
Davis, M., Malcolm, S., Hall, A. & Marshall, C. J. EMBO J. 2, 2281–2283 (1983).
Marugg, J. E. et al. Nucleic Acids Res. 12, 8639–8651 (1984).
Janssen, J. W. G., Steenvoorden, A. C. M., Collard, J. & Nusse, R. Cancer Res. (in the press).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Bos, J., Toksoz, D., Marshall, C. et al. Amino-acid substitutions at codon 13 of the N-ras oncogene in human acute myeloid leukaemia. Nature 315, 726–730 (1985). https://doi.org/10.1038/315726a0
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1038/315726a0
This article is cited by
-
Worse prognosis of KRASc.35 G > A mutant metastatic colorectal cancer (MCRC) patients treated with intensive triplet chemotherapy plus bevacizumab (FIr-B/FOx)
BMC Medicine (2013)
-
Cytogenetic and molecular predictors of response in patients with myeloid malignancies without del[5q] treated with lenalidomide
Journal of Hematology & Oncology (2012)
-
PHF6 mutations in adult acute myeloid leukemia
Leukemia (2011)
-
Krüppel-like factor 5 is a crucial mediator of intestinal tumorigenesis in mice harboring combined Apc Min and KRASV 12mutations
Molecular Cancer (2010)
-
Ras oncogenes: split personalities
Nature Reviews Molecular Cell Biology (2008)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.