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Reconstruction of Three Dimensional Structures from Electron Micrographs

Abstract

General principles are formulated for the objective reconstruction of a three dimensional object from a set of electron microscope images. These principles are applied to the calculation of a three dimensional density map of the tail of bacteriophage T4.

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References

  1. Klug, A., and Berger, J. E., J. Mol. Biol., 10, 565 (1964).

    Article  CAS  Google Scholar 

  2. Klug, A., and De Rosier, D. J., Nature, 212, 29 (1966).

    Article  CAS  ADS  Google Scholar 

  3. Klug, A., Crick, F. H. C., and Wyckoff, H. W., Acta Cryst., 11, 199 (1958).

    Article  CAS  Google Scholar 

  4. Leith, E. N., and Upatnieks, J., J. Opt. Soc. Amer., 54, 1295 (1964).

    Article  ADS  Google Scholar 

  5. Stroke, G. W., App. Phys. Lett., 6, 201 (1965).

    Article  ADS  Google Scholar 

  6. Bragg, W. L., Nature, 166, 399 (1950).

    Article  CAS  Google Scholar 

  7. Vander Lugt, A., Trans. IREE, IT-10, 139 (1964).

  8. Brenner, S., Streisinger, G., Horne, R. W., Champe, S. P., Barnett, L., Benzer, S., and Rees, M. W., J. Mol. Biol., 1, 281 (1959).

    Article  CAS  Google Scholar 

  9. Finch, J. T., Klug, A., and Nermut, M. V., J. Cell Sci., 2, (December, 1967).

  10. Moody, M. F., J. Mol. Biol., 25, 201 (1967).

    Article  CAS  Google Scholar 

  11. Krimm, S., and Anderson, T. F., J. Mol. Biol., 27, 197 (1967).

    Article  CAS  Google Scholar 

  12. See for example, Kellenberger, E., and Boy de la Tour, E., J. Ultrastruct. Res., 11, 545 (1964).

    Article  CAS  Google Scholar 

  13. Williams, R. C., and Fraser, D., Virology, 2, 289 (1956).

    Article  CAS  Google Scholar 

  14. Hall, C. E., Introduction to Electron Microscopy ch. 9 (McGraw-Hill, New York, 1953).

    MATH  Google Scholar 

  15. Heidenreich, R. D., Fundamentals of Transmission Electron Microscopy, ch. 1 (Interscience, London, 1964).

    Google Scholar 

  16. Various contributions in Symp. on Quantitative Electron Microscopy (edit. by Bahr, G. F., and Zeitler, E. H.) (The Williams and Wilkins Company, Baltimore, 1965).

  17. Franklin, R. E., and Klug, A., Acta Cryst., 8, 777 (1955).

    Article  CAS  Google Scholar 

  18. Finch, J. T., and Klug, A., J. Mol. Biol., 15, 344 (1966).

    Article  CAS  Google Scholar 

  19. Klug, A., and Finch, J. T., J. Mol. Biol. (in the press, 1968).

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DE ROSIER, D., KLUG, A. Reconstruction of Three Dimensional Structures from Electron Micrographs. Nature 217, 130–134 (1968). https://doi.org/10.1038/217130a0

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