Nature 362, 31 - 38 (04 March 1993); doi:10.1038/362031a0

Cloning and expression of an inwardly rectifying ATP-regulated potassium channel

Kevin Ho^*, Colin G. Nichols^†, W. Jonathan Lederer^‡, Jonathan Lytton^*, Peter M. Vassilev^§, Marie V. Kanazirska^§ & Steven C. Hebert^*

^* Harvard Center for the Study of Kidney Disease, Harvard Medical School and Laboratory of Molecular Physiology and Biophysics, Renal Division, Department of Medicine, Brigham and Women's Hospital, 75 Francis Street, Boston, Massachusetts 02115, USA
^† Department of Cell Biology and Physiology, Washington University School of Medicine, Washington University Medical Center, St Louis, Missouri 63110, USA
^‡ Department of Physiology, University of Maryland, Baltimore, Maryland 21201, USA
^§ Endocrine and Hypertension Division, Department of Medicine, Brigham and Women's Hospital and Harvard Medical School, Boston, Massachusetts 02115, USA
To whom correspondence should be addressed.

A complementary DNA encoding an ATP-regulated potassium channel has been isolated by expression cloning from rat kidney. The predicted 45K protein, which features two potential membrane-spanning helices and a proposed ATP-binding domain, represents a major departure from the basic structural design characteristic of voltage-gated and second messenger-gated ion channels. But the presence of an H5 region, which is likely to form the ion conduction pathway, indicates that the protein may share a common origin with voltage-gated potassium channel proteins.

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