Abstract

The human genome is by far the largest genome to be sequenced, and its size and complexity present many challenges for sequence assembly. The International Human Genome Sequencing Consortium constructed a map of the whole genome to enable the selection of clones for sequencing and for the accurate assembly of the genome sequence. Here we report the construction of the whole-genome bacterial artificial chromosome (BAC) map and its integration with previous landmark maps and information from mapping efforts focused on specific chromosomal regions. We also describe the integration of sequence data with the map.

1. Washington University School of Medicine, Genome Sequencing Center, Department of Genetics, 4444 Forest Park Boulevard, St. Louis, Missouri 63108, USA
2. Wellcome Trust Genome Campus, Hinxton, Cambridge, CB10 1SA, UK
3. National Center for Biotechnology Information, National Institutes of Health, Bethesda, Maryland 20894, USA
4. National Human Genome Research Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
5. Department of Molecular Genetics, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, New York 10461, USA
6. Baylor College of Medicine, Human Genome Sequencing Center, Houston, Texas, USA;
7. University of Texas, San Antonio, Texas, USA
8. Roswell Park Cancer Institute, Buffalo, New York 14263, USA
9. Multimegabase Sequencing Center, Institute for Systems Biology, Seattle, Washington 98105, USA
10. Division of Human Biology, Fred Hutchinson Cancer Research Institute, Seattle, Washington 98109, USA
11. Department of Pediatrics, The Children's Hospital of Philadelphia, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA;
12. Genetics Department, Medicine Branch, National Cancer Institute, Washington DC, USA
13. Genoscope, Centre National de Séquencage, 2 Rue Gaston Crémieux, CP 5706, 91057 Evry, France
14. US DOE Joint Genome Institute, Walnut Creek, California, USA
15. Stanford Human Genome Center and Department of Genetics, Stanford University School of Medicine, Stanford, California 94305, USA
16. Howard Hughes Medical Institute, University of California, Santa Cruz, Santa Cruz, California 95064, USA;
17. Department of Computer Science, University of California, Santa Cruz, Santa Cruz, California 95064, USA;
18. Department of Biology, University of California, Santa Cruz, Santa Cruz, California 95064, USA;
19. Department of Mathematics, University of California, Santa Cruz, Santa Cruz, California 95064, USA
20. British Columbia Cancer Research Centre, 600 West 10th Avenue, Room 3427, Vancouver, British Columbia V5Z 4E6, Canada
21. Dept. of Genome Analysis, Institute of Molecular Biotechnology, Beutenbergstrasse 11, D-07745 JENA, Germany;
22. Institute of Human Genetics, University of Kiel, Germany
23. Departments of Human Genetics and Pediatrics, University of California, Los Angeles, California, USA
24. RIKEN Genomic Sciences Center, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan
25. Department of Molecular Biology, Keio University School of Medicine, 35 Shinanomachi Shinjuku-ku, Tokyo 160-8582, Japan
26. Max Planck Institute for Molecular Genetics, Ihnestrasse 73, D-14195, Berlin, Germany;
27. Abteilung Molekulare Genomanalyse, Deutsches Krebstorschungszentrum, Im Neuenheimer Feld 280, 69120, Heidelberg, Germany

Correspondence to: John D. McPherson¹ Correspondence and requests for materials should be addressed to J.D.M. (e-mail: Email: jmcphers@watson.wustl.edu).