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Molecular structure of F-actin and location of surface binding sites

Nature volume 348, pages 217–221 (1990)Cite this article

Abstract

Comparisons of three-dimensional maps of vertebrate muscle thin filaments obtained by cryo-electron microscopy and image analysis, reveal the molecular structure of F-actin, the location of the C terminus of the monomer and the positions of the binding sites of tropomyosin, the myosin head and the N-terminal portion of the myosin Al light chain on the filament. These data provide strong constraints for evaluating models built from the atomic structure of the monomer and the subsequent identification of molecular contacts.

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References

  1. Moore, P. B., Huxley, H. E. & DeRosier, D. J. J. molec. Biol. 50, 279–295 (1970).

    Article  CAS  Google Scholar 

  2. Taylor, K. A. & Amos, L. A. J. molec. Biol. 147, 297–324 (1981).

    Article  CAS  Google Scholar 

  3. Toyoshima, C. & Wakabayashi, T. J. Biochem. 97, 219–243 (1985).

    Article  CAS  Google Scholar 

  4. Toyoshima, C. & Wakabayashi, T. J. Biochem. 97, 245–263 (1985).

    Article  CAS  Google Scholar 

  5. Vibert, P. & Craig, R. J. molec. Biol. 157, 299–319 (1982).

    Article  CAS  Google Scholar 

  6. O'Brien, E. J., Couch, J., Johnson, G. R. P. & Morris, E. P. in Actin: Structure and Function in Muscle and Non-Muscle Cells (eds dosRemedios, C. G. & Barden, J. A.) 3–15 (Academic, Australia, 1983).

    Google Scholar 

  7. Trinick, I. Cooper, J., Seymour, J. & Egelman, E. H. J. Microsc. 141, 349–360 (1986).

    Article  CAS  Google Scholar 

  8. Millonig, R., Sütterlin, R., Engel, A., Pollard, T. D. & Aebi, U. in Springer Series in Biophysics. Vol. 3, Cytoskeletal and Extracellular Proteins (eds Aebi, U. & Engel, J.) 51–53 (Springer-Verlag, Berlin, 1989).

    Book  Google Scholar 

  9. Wakabayashi, T., Huxley, H. E., Amos, L. A. & Klug, A. J. molec. Biol. 93, 477–497 (1975).

    Article  CAS  Google Scholar 

  10. Egelman, E. H. J. Muscle Res. Cell Motil. 6, 129–151 (1985).

    Article  CAS  Google Scholar 

  11. Milligan, R. A. & Flicker, P. F. J. Cell Biol. 105, 29–39 (1987).

    Article  CAS  Google Scholar 

  12. Sakabe, N. et al. J. Biochem. 93, 299–302 (1983).

    Article  CAS  Google Scholar 

  13. Schutt, C. E., Lindberg, U., Myslik, J. & Strauss, N. J. molec. Biol. 209, 735–746 (1989).

    Article  CAS  Google Scholar 

  14. Kabsch, W., Mannherz, H. G., Suck, D., Pai, E. F. & Holmes, K. C. Nature 347, 37–44 (1990).

    Article  CAS  ADS  Google Scholar 

  15. DeRosier, D. J. & Moore, P. B. J. molec. Biol. 52, 355–369 (1970).

    Article  CAS  Google Scholar 

  16. Sutoh, K. Biochemistry 21, 3654–3661 (1982).

    Article  CAS  Google Scholar 

  17. Trayer, I. P., Trayer, H. R. & Levine, B. A. Eur. J. Biochem. 164, 259–266 (1987).

    Article  CAS  Google Scholar 

  18. Safer, D., Bolinger, L. & Leigh, J. S. Jr J. Inorg. Biochem. 26, 77–91 (1986).

    Article  CAS  Google Scholar 

  19. Erickson, H. P. J. molec. Biol. 206, 465–474 (1989).

    Article  CAS  Google Scholar 

  20. Kasprzak, A. A., Takashi, R. & Morales, M. F. Biochemistry 27, 4512–4522 (1988).

    Article  CAS  Google Scholar 

  21. Taylor, D. L., Reidler, K., Spudich, J. A. & Stryer, L. J. Cell Biol. 89, 362–367 (1981).

    Article  CAS  Google Scholar 

  22. Elzinga, M. & Phelan, J. J. Proc. natn. Acad. Sci. U.S.A. 81, 6599–6602 (1984).

    Article  CAS  ADS  Google Scholar 

  23. Sutoh, K. & Mabuchi, I. Biochemistry 23, 6757–6761 (1984).

    Article  CAS  Google Scholar 

  24. Mornet, D., Bonet, A., Audemard, E. & Bonicel, J. J. Muscle Res. Cell Motil. 10, 10–24 (1989).

    Article  CAS  Google Scholar 

  25. Mornet, D., Bertrand, R., Pantel, P., Audemard, E. & Kassab, R. Nature 292, 301–306 (1981).

    Article  CAS  ADS  Google Scholar 

  26. Chaussepied, P. & Kasprzak, A. A. Nature 342, 950–953 (1989).

    Article  CAS  ADS  Google Scholar 

  27. Chalovitch, J. M., Stein, L. A., Greene, L. E. & Eisenberg, E. Biochemistry 23, 4885–4889 (1984).

    Article  Google Scholar 

  28. Holmes, K. C., Popp, D., Gebhard, W. & Kabsch, W. Nature 347, 44–49 (1990).

    Article  CAS  ADS  Google Scholar 

  29. Phillips, G. N., Jr, Fillers, J. P. & Cohen, C. J. molec. Biol. 192, 111–131 (1986).

    Article  CAS  Google Scholar 

  30. Herzberg, O. & James, M. N. G. J. molec. Biol. 203, 761–779 (1988).

    Article  CAS  Google Scholar 

  31. Rayment, I. & Winkelmann, D. A. Proc. natn. Acad. Sci. U.S.A. 81, 4378–4380 (1984).

    Article  CAS  ADS  Google Scholar 

  32. Spudich, J. A. & Watt, S. J. biol. Chem. 246, 4866–4871 (1971).

    CAS  PubMed  Google Scholar 

  33. Pardee, J. D. & Spudich, J. A. Meth. Enzym. 85, 164–181 (1982).

    Article  CAS  Google Scholar 

  34. Weeds, A. G. & Taylor, R. S. Nature 257, 54–56 (1975).

    Article  CAS  ADS  Google Scholar 

  35. Ebashi, S. & Ebashi, F. J. Biochem. 55, 604–613 (1964).

    Article  CAS  Google Scholar 

  36. Safer, D. Analyt. Biochem. 178, 32–37 (1989).

    Article  CAS  Google Scholar 

  37. Goodsell, D. S., Mian, I. S. & Olson, A. J. J. molec. Graphics 7, 41–47 (1989).

    Article  CAS  Google Scholar 

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Author notes

  1. D. Safer: Department of Anatomy, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA

Authors and Affiliations

  1. Department of Molecular Biology, Research Institute of Scripps Clinic, 10666 North Torrey Pines Road, La Jolla, California, 92037, USA

    R. A. Milligan, M. Whittaker & D. Safer

Authors
  1. R. A. Milligan
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  2. M. Whittaker
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  3. D. Safer
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Milligan, R., Whittaker, M. & Safer, D. Molecular structure of F-actin and location of surface binding sites. Nature 348, 217–221 (1990). https://doi.org/10.1038/348217a0

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