Nature 344, 742 - 746 (19 April 1990); doi:10.1038/344742a0

2-Microglobulin deficient mice lack CD4^-8⁺ cytolytic T cells

Maarten Zijlstra, Mark Bix^*, Neil E. Simister, Janet M. Loring, David H. Raulet^* & Rudolf Jaenisch

Whitehead Institute for Biomedicai Research, Nine Cambridge Center, and Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02142, USA
^* Center for Cancer Research and Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02142, USA

Mice homozygous for a 2-microglobulin gene disruption do not express any detectable 2-ITL protein. They express little if any functional major histocompatibility complex (MHC) class I antigen on the cell surface yet are fertile and apparently healthy. They show a normal distribution of , CD4⁺8⁺ and CD4⁺8^- cells, but have no mature CD4^-8⁺ cells and are defective in CD4^-8⁺ cell-mediated cytotoxicity. Our results strongly support earlier evidence that MHC class I molecules are crucial for positive selection of T cell antigen receptor ⁺ CD4^-8⁺ T cells in the thymus and call into question the non-immune functions that have been ascribed to MHC class I molecules.

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