Nature 342, 762 - 766 (14 December 1989); doi:10.1038/342762a0

Primary structure and functional expression from complementary DNA of the rod photoreceptor cyclic GMP-gated channel

U. Benjamin Kaupp^*, Tetsuhiro Niidome, Tsutomu Tanabe, Shoichi Terada, Wolfgang Bönigk^*, Walter Stühmer^†, Neil J. Cook^‡£, Kenji Kangawa^§, Hisayuki Matsuo^§, Tadaaki Hirose, Takashi Miyata^¶ & Shosaku Numa

Departments of Medical Chemistry and Molecular Genetics, Kyoto University Faculty of Medicine, Kyoto 606, Japan
^*Institut für Biologische Informationsverarbeitung, Kernforschungsanlage, D-5170 Jülich, FRG
^†Max-Planck-lnstitut für biophysikalische Chemie, D-3400 Göttingen, FRG
^‡Abteilung Biophysik, Universität Osnabrück, D-4500 Osnabrück, FRG
^§Department of Biochemistry, Miyazaki Medical College, Miyazaki 889-16, Japan
Pharmaceutical Institute, Keio University School of Medicine, Tokyo 160, Japan
^¶Department of Biology, Kyushu University Faculty of Science, Fukuoka 812, Japan
^£Present address: Max-Planck-lnstitut für Biophysik, Abteilung Molekulare Membranbiologie, D-6000 Frankfurt 71, FRG

The complete amino-acid sequence of the cyclic GMP-gated channel from bovine retinal rod photo-receptors, deduced by cloning and sequencing its complementary DNA, shows that the protein contains several putative transmembrane segments, followed by a region that is similar to the cyclic GMP-binding domains of cyclic GMP-dependent protein kinase. Expression of the complementary DNA produces cyclic GMP-gated channel activity in Xenopus oocytes.

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