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A fusion protein required for vesicle-mediated transport in both mammalian cells and yeast

Nature volume 339, pages 355–359 (1989)Cite this article

Abstract

A protein sensitive toN-ethylmaleimide catalyses the fusion of transport vesicles with Golgi cisternae in a mammalian cell-free system. By cloning and sequencing its gene from Chinese hamster ovary cells and by use of in vitro assays, we show that this fusion protein is equivalent to the SEC18 gene product of the yeast Saccharomyces cerevisiae,known to be essential for vesicle-mediated transport from the endoplasmic reticulum to the Golgi apparatus. The mechanism of vesicular fusion is thus highly conserved, both between species and at different stages of transport.

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Author notes

  1. Marc R. Block

    Present address: Laboratoire de Physiologie Cellulaire, Universite Joseph Fourier, B.P. 53X, 38041, Grenoble CEDEX, France

  2. Axel Ullrich

    Present address: Max-Planck Institut fur Biochemie, 8033, Martinsried, FRG

Authors and Affiliations

  1. Department of Biology, Princeton University, Princeton, New Jersey, 08540, USA

    Duncan W. Wilson, Gregory C. Flynn & James E. Rothman

  2. Department of Biochemistry, Stanford University, Stanford, California, 94305, USA

    Celeste A. Wilcox & Marc R. Block

  3. Genentech Inc., 460 Point San Bruno Boulevard, South San Francisco, California, 94080, USA

    Ellson Chen, Wun-Jing Kuang, William J. Henzel & Axel Ullrich

Authors
  1. Duncan W. Wilson
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  2. Celeste A. Wilcox
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  3. Gregory C. Flynn
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  4. Ellson Chen
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  5. Wun-Jing Kuang
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  6. William J. Henzel
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  7. Marc R. Block
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  9. James E. Rothman
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Wilson, D., Wilcox, C., Flynn, G. et al. A fusion protein required for vesicle-mediated transport in both mammalian cells and yeast. Nature 339, 355–359 (1989). https://doi.org/10.1038/339355a0

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