Abstract
STEROID hormone receptors activate specific gene transcription by binding as hormone-receptor complexes to DNA enhancer elements termed hormone responsive elements (refs 1, 2 and references therein). A highly conserved 66-amino-acid region of the oestrogen and glucocorticoid receptors which corresponds to part of the receptor DNA-binding domain (region C) determines the specificity of target gene recognition3–5. This region contains two subregions (CI and CII), encoded in two separate exons (refs 1, 7 and references therein), that are analogous to the 'zinc fingers' of the transcription factor TFIIIA (reviewed in ref. 6). The N-terminal CI finger determines the recognition specificity of the hormone responsive element8. A chimaeric oestrogen receptor, in which the CI finger is replaced with the corresponding glucocorticoid receptor CI finger region, activates transcription from a reporter gene containing a glucocorticoid-responsive element, but not from a reporter gene containing an oestrogen-responsive element8. We report here that three amino acids located at the C-terminal side of the oestrogen receptor CI finger play a key part in this specificity.
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Mader, S., Kumar, V., de Verneuil, H. et al. Three amino acids of the oestrogen receptor are essential to its ability to distinguish an oestrogen from a glucocorticoid-responsive element. Nature 338, 271–274 (1989). https://doi.org/10.1038/338271a0
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DOI: https://doi.org/10.1038/338271a0
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