Nature 333, 742 - 746 (23 June 1988); doi:10.1038/333742a0

Tolerance in T-cell-receptor transgenic mice involves deletion of nonmature CD4⁺8⁺ thymocytes

Pawel Kisielow^*†, Horst Blüthmann^‡, Uwe D. Staerz^*, Michael Steinmetz^‡ & Harald von Boehmer^*

^*Basel Institute for Immunology, CH-4005 Basel, Switzerland
^‡Central Research Units, F. Hoffmann-La Roche & Co. Ltd, CH-4002 Basel, Switzerland
^† Permanent address: The Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wroclaw, Poland.

The mechanism of self-tolerance is studied in T-cell-receptor transgenic mice expressing a receptor in many of their T cells for the male (H−Y) antigen in the context of class I H−2D^b MHC antigens. Autospecific T cells are deleted in male mice. The deletion affects only transgene-expressing cells with a relatively high surface-density of CDS molecules, including nonmature CD4⁺CD8⁺ thymocytes, and is not caused by anti-idiotype cells.

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