Abstract
The exoskeletons of sea urchins are composed of magnesiumbearing calcite. Individual test plates and spines behave as single crystals in polarized light or when examined by X-ray diffraction1–3. They do not, however, cleave like inorganic calcite crystals along the {104} hexagonal cleavage planes, but have conchoidal fracture surfaces reminiscent of amorphous glass. Discussion of this paradox revolves around whether the phase is monocrystalline2,4,5, multicrystalline6, or some combination thereof7, but provides no explanation for the phenomenon. To address this question we grew crystals of calcite in the presence of acidic glycoproteins extracted from within the mineralized hard parts of sea-urchin tests8–10. As a control we used analogous proteins from the calcitic layer of a mollusc shell which are known to be nucleators of calcite when adsorbed on a rigid substrate, but inhibitors when in solution11–13. We show that the sea urchin, but not the mollusc macromolecules selectively adsorb onto specific calcite crystal planes and with continued crystal growth are occluded inside the solid phase. These synthetic crystals fracture with a conchoidal cleavage similar to that observed in sea-urchin calcite. Thus intracrystalline proteins may be responsible for this phenomenon in biology and the manner in which they affect the mechanical properties of the crystals may also have interesting implications to the materials sciences.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Raup, D. M. in Physiology of Echinodermata. (ed. Boolootian, R. A.) 379–395 (Wiley, New York, 1966).
Blake, D. F., Peacor, D. R. & Allard, L. F. Micron, microscopia Acta 15(2), 85–90 (1984).
Blake, D. F. & Peacor, D. R. SEM III, 321–328 (1981).
Nissen, H. Science 166, 1150–1152 (1969).
Donnay, G. & Pawson, D. L. Science 166, 1147–1150 (1969).
O'Neill, P. L. Science 213, 646–648 (1981).
Towe, K. Science 157, 1048–1050 (1967).
Weiner, S. J. exp. Zool. 234, 7–15 (1985).
Swift, D. M., Sikes, C. S. & Wheeler, A. P. J. exp. Zool. 240, 65–73 (1986).
Benson, S. C., Benson, N. C. & Wilt, F. J. Cell Biol. 102, 1878–1886 (1986).
Addadi, L. & Weiner, S. Proc. natn. Acad. Sci. U.S.A. 82, 4110–4114 (1985).
Addadi, L. & Weiner, S. Molec. Crystallogr. liq. Crystallogr. 134, 305–322 (1986).
Addadi, L., Moradian, J., Shay, E., Maroudas, N. G. & Weiner, S. Proc. natn. Acad. Sci. U.S.A. 84, 2732–2736 (1987).
Addadi, L. et al. Nature 296, 21–26 (1982).
Emlet, R. B. Biol. Bull. 163, 264–275 (1982).
Weiner, S. Biochemistry 22, 4139–4145 (1983).
Worms, D. & Weiner, S. J. exp. Zool. 237, 11–20 (1986).
Gray, W. R. Meth. Enzym. 11, 139–151 (1967).
Weiner, S. & Tishbee, A. J. Chromat. 213, 501–506 (1981).
Jones, B. M., Paabo, S. & Stein, S. J. liq. Chromatogr. 4, 565–586 (1981).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Herman, A., Addadi, L. & Weiner, S. Interactions of sea-urchin skeleton macromolecules with growing calcite crystals— a study of intracrystalline proteins. Nature 331, 546–548 (1988). https://doi.org/10.1038/331546a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/331546a0
This article is cited by
-
Soil improvement by microbially induced calcite precipitation (MICP): a review about mineralization mechanism, factors, and soil properties
Arabian Journal of Geosciences (2022)
-
Effects of seawater pCO2 on the skeletal morphology of massive Porites spp. corals
Marine Biology (2022)
-
Strategies for simultaneous strengthening and toughening via nanoscopic intracrystalline defects in a biogenic ceramic
Nature Communications (2020)
-
Hydroxyl-rich macromolecules enable the bio-inspired synthesis of single crystal nanocomposites
Nature Communications (2019)
-
Direct observation of mineral–organic composite formation reveals occlusion mechanism
Nature Communications (2016)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.