Nature 323, 411 - 416 (02 October 1986); doi:10.1038/323411a0

Cloning, sequencing and expression of complementary DNA encoding the muscarinic acetylcholine receptor

Tai Kubo^*, Kazuhiko Fukuda^*, Atsushi Mikami^*, Akito Maeda^*, Hideo Takahashi^*, Masayoshi Mishina^*, Tatsuya Haga^†, Kazuko Haga^†, Arata Ichiyama^†, Kenji Kangawa^‡, Masayasu Kojima^‡, Hisayuki Matsuo^‡, Tadaaki Hirose^§ & Shosaku Numa^*

^*Departments of Medical Chemistry and Molecular Genetics, Kyoto University Faculty of Medicine, Kyoto 606, Japan
^†Department of Biochemistry, Hamamatsu University School of Medicine, Hamamatsu 431-31, Japan
^‡Department of Biochemistry, Miyazaki Medical College, Miyazaki 889-16, Japan
^§Pharmaceutical Institute, Keio University School of Medicine, Tokyo 160, Japan
To whom correspondence should be addressed.

Cloning and sequence analysis of DNA complementary to porcine cerebral messenger RNA encoding the muscarinic acetylcholine receptor predict the complete amino-acid sequence of this protein. Expression of the complementary DNA produces functional muscarinic receptor in Xenopus oocytes. The muscarinic receptor is homologous with the -adrenergic receptor and rhodopsin in both amino-acid sequence and suggested transmembrane topography.

References

1.	Burgen, A. S. V. Trends pharmac. Sci. Suppl. 5, 1−3 (1984).
2.	Brown, D. Nature 319, 358−359 (1986). | Article | PubMed | ISI | ChemPort |
3.	Gilman, A. G. Cell 36, 577−579 (1984). | Article | PubMed | ISI | ChemPort |
4.	Hammer, R., Berrie, C. P., Birdsall, N. J. M., Burgen, A. S. V. & Hulme, E. C. Nature 283, 90−92 (1980). | PubMed | ISI | ChemPort |
5.	Birdsall, N. J. M. & Hulme, E. C. Trends pharmac. Sci. 4, 459−463 (1983). | Article | ISI | ChemPort |
6.	Watson, M. et al. Trends pharmac. Sci. Suppl. 5, 46−55 (1986).
7.	Haga, K. & Haga, T. J. biol. Chem. 260, 7927−7935 (1985). | PubMed | ISI | ChemPort |
8.	Haga, K. et al. Nature 316, 731−733 (1985). | PubMed | ISI | ChemPort |
9.	Haga, K., Haga, T. & Ichiyama, A. J. biol. Chem. 261, 10133−10140 (1986). | PubMed | ChemPort |
10.	Hewick, R. M., Hunkapiller, M. W., Hood, L. E. & Dreyer, W. J. J. biol. Chem. 256, 7990−7997 (1981). | PubMed | ISI | ChemPort |
11.	Okayama, H. & Berg, P. Molec. cell Biol. 2, 161−170 (1982). | PubMed | ISI | ChemPort |
12.	Grantham, R., Gautier, C., Gouy, M., Jacobzone, M. & Mercier, R. Nucleic Acids Res. 9, r43−r74 (1981). | PubMed | ISI | ChemPort |
13.	Maxam, A. M. & Gilbert, W. Meth. Enzym. 65, 499−560 (1980). | Article | PubMed | ChemPort |
14.	Kozak, M. Nucleic Acids Res. 12, 857−872 (1984). | PubMed | ISI | ChemPort |
15.	Shirakawa, O., Kuno, T. & Tanaka, C. Biochem. biophys. Res. Commun. 115, 814−819 (1983). | PubMed | ISI | ChemPort |
16.	Grefrath, S. P. & Reynolds, J. A. Proc. natn. Acad. Sci. U.S.A. 71, 3913−3916 (1974). | ChemPort |
17.	Peterson, G. L., Rosenbaum, L. C., Broderick, D. J. & Schimerlik, M. I. Biochemistry 25, 3189−3202 (1986). | PubMed | ISI | ChemPort |
18.	Proudfoot, N. J. & Brownlee, G. G. Nature 263, 211−214 (1976). | PubMed | ISI | ChemPort |
19.	Dixon, R. A. F. et al. Nature 321, 75−79 (1986). | Article | PubMed | ISI | ChemPort |
20.	Green, S. et al. Nature 320, 134−139 (1986). | Article | PubMed | ISI | ChemPort |
21.	Kyte, J. & Doolittle, R. F. J. molec. Biol. 157, 105−132 (1982). | PubMed | ISI | ChemPort |
22.	Engelman, D. M., Goldman, A. & Steitz, T. A. Meth. Enzym. 88, 81−88 (1982). | Article | ISI | ChemPort |
23.	Ovchinnikov, Y. A. FEBS Lett. 148, 179−191 (1982). | Article | PubMed | ISI | ChemPort |
24.	Hargrave, P. A. et al. Biophys. Struct. Mech. 9, 235−244 (1983). | PubMed | ISI | ChemPort |
25.	Nathans, J. & Hogness, D. S. Cell 34, 807−814 (1983). | Article | PubMed | ISI | ChemPort |
26.	Toh, H., Hayashida, H. & Miyata, T. Nature 305, 827−829 (1983). | PubMed | ISI | ChemPort |
27.	Dayhoff, M. O., Schwartz, R. M. & Orcutt, B. C. in Atlas of Protein Sequence and Structure Vol. 5 Suppl. 3 (ed. Dayhofi, M. O.) 345−352 (National Biomedical Research Foundation, Silver Spring, Maryland, 1978).
28.	Henderson, R. & Unwin, P. N. T. Nature 257, 28−32 (1975). | PubMed | ISI | ChemPort |
29.	Khorana, H. G. et al. Proc. natn. Acad. Sci. U.S.A. 76, 5046−5050 (1979). | ChemPort |
30.	Ovchinnikov, Y. A., Abdulaev, N. G., Feigina, M. Y., Kiselev, A. V. & Lobanov, N. A. FEBS Lett. 100, 219−224 (1979). | Article | PubMed | ISI | ChemPort |
31.	Hargrave, P. A. Prog. Retinal Res. 1, 1−51 (1982). | Article | ChemPort |
32.	Blobel, G. & Dobberstein, B. J. Cell Biol. 67, 852−862 (1975). | Article | PubMed | ISI | ChemPort |
33.	Hubbard, S. C. & Ivatt, R. J. A. Rev. Biochem. 50, 555−583 (1981). | Article | ISI | ChemPort |
34.	Benovic, J. L., Mayor, F. Jr, Somers, R. L., Caron, M. G. & Lefkowitz, R. J. 321, 869−872 (1986). | ChemPort |
35.	Krebs, E. G. & Beavo, J. A. A. Rev. Biochem. 48, 923−959 (1979). | Article | ISI | ChemPort |
36.	Melton, D. A. et al. Nucleic Acids Res. 12, 7035−7056 (1984). | PubMed | ISI | ChemPort |
37.	Kusano, K., Miledi, R. & Stinnakre, J. J. Physiol., Lond. 328, 143−170 (1982). | PubMed | ISI | ChemPort |
38.	Dascal, N., Landau, E. M. & Lass, Y. J. Physiol., Lond. 352, 551−574 (1984). | PubMed | ISI | ChemPort |
39.	Sugiyama, H., Hisanaga, Y. & Hirono, C. Brain Res. 338, 346−350 (1985). | Article | PubMed | ISI | ChemPort |
40.	Mishina, M. et al. Nature 307, 604−608 (1984). | PubMed | ISI | ChemPort |
41.	Barnard, E. A., Miledi, R. & Sumikawa, K. Proc. R. Soc. B215, 241−246 (1982). | ISI | ChemPort |
42.	Barish, M. E. J. Physiol., Lond. 342, 309−325 (1983). | PubMed | ISI | ChemPort |
43.	Yamamura, H. I. & Snyder, S. H. Proc. natn. Acad. Sci. U.S.A. 71, 1725−1729 (1974). | ChemPort |
44.	Hulme, E. C., Birdsall, N. J. M., Burgen, A. S. V. & Mehta, P. Molec. Pharmac. 14, 737−750 (1978). | ISI | ChemPort |
45.	Berrie, C. P. et al. Trends pharmac. Sci. Suppl. 7, 8−13 (1986).
46.	Harden, T. K. et al. Trends pharmac. Sci. Suppl. 7, 14−18 (1986).
47.	Fung, B. K.-K., Hurley, J. B. & Stryer, L. Proc. natn. Acad. Sci. U.S.A. 78, 152−156 (1981). | ChemPort |
48.	Kanaho, Y. et al. J. biol. Chem. 259, 7378−7381 (1984). | PubMed | ISI | ChemPort |
49.	Nathans, J., Thomas, D. & Hogness, D. S. Science 232, 193−202 (1986). | PubMed | ISI | ChemPort |
50.	Nakayama, N., Miyajima, A. & Arai, K. EMBO J. 4, 2643−2648 (1985). | ISI | ChemPort |
51.	Chirgwin, J. M., Przybyla, A. E., MacDonald, R. J. & Rutter, W. J. Biochemistry 18, 5294−5299 (1979). | PubMed | ISI | ChemPort |
52.	Aviv, H. & Leder, P. Proc. natn. Acad. Sci. U.S.A. 69, 1408−1412 (1972). | ChemPort |
53.	Boyer, H. W. & Roulland-Dussoix, D. J. molec. Biol. 41, 459−472 (1969). | PubMed | ISI | ChemPort |
54.	Wahl, G. M., Padgett, R. A. & Stark, G. R. J. biol. Chem. 254, 8679−8689 (1979). | PubMed | ISI | ChemPort |
55.	Hanahan, D. & Meselson, M. Gene 10, 63−67 (1980). | Article | PubMed | ISI | ChemPort |
56.	Ito, H., Ike, Y., Ikuta, S. & Itakura, K. Nucleic Acids Res. 10, 1755−1769 (1982). | PubMed | ISI | ChemPort |
57.	Konarska, M. M., Padgett, R. A. & Sharp, P. A. Cell 38, 731−736 (1984). | Article | PubMed | ISI | ChemPort |
58.	Gurdon, J. B. The Control of Gene Expression in Animal Development (Clarendon, Oxford, 1974).
59.	Sakmann, B. et al. Nature 318, 538−543 (1985). | Article | PubMed | ISI | ChemPort |
60.	Nukada, T., Haga, T. & Ichiyama, A. Molec. Pharmac. 24, 366−373 (1983). | ISI | ChemPort |
61.	McMaster, G. & Carmichael, G. G. Proc. natn. Acad. Sci. U.S.A. 74, 4835−4838 (1977). | ChemPort |
62.	Thomas, P. S. Proc. natn. Acad. Sci. U.S.A. 77, 5201−5205 (1980). | ChemPort |
63.	Mishina, M. et al. Nature 321 406−411 (1986). | Article | PubMed | ISI | ChemPort |
64.	Noller, H. F. A. Rev. Biochem. 53, 119−162 (1984). | Article | ISI | ChemPort |