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Molecular cloning and expression of cDNA for human granulocyte colony-stimulating factor

Nature volume 319, pages 415–418 (1986)Cite this article

Abstract

Granulocyte colony-stimulating factor (G-CSF) is a member of the CSF family of hormone-like glycoproteins that regulate haematopoietic cell proliferation and differentiation1,2, and G-CSF almost exclusively stimulates the colony formation of granulocytes from committed precursor cells in semi-solid agar culture2. Recently, Nomura et al.3 have established a human squamous carcinoma cell line (designated CHU-2) from a human oral cavity tumour which produces large quantities of CSF constitutively, and the CSF produced by CHU-2 cells has been purified to homogeneity from the conditioned medium. We have now determined the partial amino-acid sequence of the purified G-CSF protein, and by using oligonucleotides as probes, have isolated several clones containing G-CSF complementary DNA from the cDNA library prepared with messenger RNA from CHU-2 cells. The complete nucleotide sequences of two of these cDNAs were determined and the expression of the cDNA in monkey COS cells gave rise to a protein showing authentic G-CSF activity. Furthermore, Southern hybridization analysis of DNA from normal leukocytes and CHU-2 cells suggests that the human genome contains only one gene for G-CSF and that some rearrangement has occurred within one of the alleles of the G-CSF gene in CHU-2 cells.

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Author notes

  1. Tatsumi Yamazaki, Osami Yamamoto, Yuichi Hirata, Naoki Kubota, Masayoshi Oheda, Hitoshi Nomura and Masayoshi Ono: New Drug Research Laboratories, Chugai Pharmaceutical Co., 3-41-8 Takada, Toshima-ku, Tokyo 171, Japan

Authors and Affiliations

  1. Institute of Medical Science, University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo, 108, Japan

    Shigekazu Nagata, Masayuki Tsuchiya, Shigetaka Asano, Yoshito Kaziro, Tatsumi Yamazaki, Osami Yamamoto, Yuichi Hirata, Naoki Kubota, Masayoshi Oheda, Hitoshi Nomura & Masayoshi Ono

Authors
  1. Shigekazu Nagata
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  2. Masayuki Tsuchiya
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  3. Shigetaka Asano
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  4. Yoshito Kaziro
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  5. Tatsumi Yamazaki
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  6. Osami Yamamoto
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  7. Yuichi Hirata
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  8. Naoki Kubota
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  9. Masayoshi Oheda
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  10. Hitoshi Nomura
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  11. Masayoshi Ono
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Nagata, S., Tsuchiya, M., Asano, S. et al. Molecular cloning and expression of cDNA for human granulocyte colony-stimulating factor. Nature 319, 415–418 (1986). https://doi.org/10.1038/319415a0

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