Abstract
A new ‘photofootprinting’ technique, which uses light to detect protein–DNA contacts as well as changes in the structure of DNA at the base pair level, has been developed and used to detect contacts between lac repressor and the lac operator in Escherichia coli cells.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Rent or buy this article
Prices vary by article type
from$1.95
to$39.95
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Galas, D. & Schmitz, A. Nucleic Acids Res. 5, 3157–3170 (1978).
Gilbert, W., Maxam, A. & Mirzabekov, A. in Control of Ribosome Synthesis (eds Kjelgaand, N. C. & Maaloe, O.) 139–148 (Munkasgaard, Copenhagen, 1976).
Siebenlist, U., Simpson, R. B. & Gilbert, W. Cell 20, 269–281 (1980).
Ogata, R. & Gilbert, W. Proc. natn. Acad. Sci. U.S.A. 74, 4973–4976 (1977).
Ross, W., Landy, A., Kikuchi, Y. & Nash, H. Cell 18, 297–307 (1978).
Van Dyke, M., Hertzberg, R. P. & Dervan, P. B. Proc. natn. Acad. Sci. U.S.A. 79, 5470–5474 (1982).
Maxam, A. M. & Gilbert, W. Proc. natn. Acad. Sci. U.S.A. 74, 560–564 (1977).
Wang, S. Y. (ed.) Photochemistry and Photobiology of Nucleic Acids Vols 1, 2 (Academic, New York, 1976).
Ciarrocchi, G. & Pedrini, A. M. J. molec. Biol. 155, 177–183 (1982).
Ciarrocchi, G. & Sutherland, B. M. Photochem. Photobiol. 38, 259–263 (1983).
Denhardt, D. T. & Kato, A. C. J. molec. Biol. 77, 479–494 (1973).
Bose, S. N., Davies, R. J. H., Sethi, S. K. & McCloskey, J. A. Science 13, 723–725 (1983).
Brown, H. C., Wheeler, O. H. & Ichikawa, K. Tetrahedron 1, 214–220 (1957).
Cerutti, P. & Miller, N. J. molec. Biol. 26, 55–66 (1967).
Kunieda, T. & Witkop, B. J. Am. chem. Soc. 93, 3493–3499 (1971).
Miller, N. & Cerutti, P. Proc. natn. Acad. Sci. U.S.A. 59, 34–38 (1968).
Green, M. & Cohen, S. S. J. biol. Chem. 225, 397–407 (1957).
Roberts, T. M., Kacichi, R. & Ptashne, M. Proc. natn. Acad. Sci. U.S.A. 76, 760–764 (1979).
Ogata, R. T. & Gilbert, W. J. molec. Biol. 132, 709–728 (1979).
Schmitz, A. & Gales, D. J. Nucleic Acids Res. 6, 111–137 (1979).
Bahl, C.P., Wu, R., Stawinsky, J. & Narang, S. A. Proc. natn. Acad. Sci. U.S.A. 74, 966–970 (1977).
Sancar, A. & Rupp, W. D. Cell 33, 249–260 (1983).
Franklin, W. A., Lo, K. M. & Haseltine, W. A. J. biol. Chem. 257, 13535–13543 (1982).
Wang, J. C. & Becherer, K. Nucleic Acids Res. 6, 1773–1790 (1983).
Meistrich, M., Lamola, A. A. & Gabbay, E. Photochem. Photobiol. 11, 169–178 (1970).
Turro, N. J. Molecular Photochemistry (Benjamin, Massachusetts, 1965).
Gilbert, W., Majors, J. & Maxam, A. in Dahlem Workshop on Chromosomes (Abakon, Berlin, 1976).
Fried, M. & Crothers, D. M. Nucleic Acids Res. 9, 6505–6526 (1981).
Musajo, L. & Rodighieno, G. Photochem. Photobiol. 11, 27–35 (1970).
Cole, R. S. Biochim. biophys. Acta 217, 30–39 (1970).
Musajo, L., Bondin, F. & Bevilacqua, R. Photochem. Photobiol. 6, 927–931 (1967).
Hansen, C. V., Shen, C.-K. J. & Hearst, J. E. Science 193, 62–64 (1976).
Cech, T. R., Potter, D. & Pardue, M. L. Cold Spring Harb. Symp. quant. Biol. 42, 191–198 (1977).
Chamberlain, M. J. A. Rev. Biochem. 43, 721–775 (1974).
Siebenlist, U. Nature 279, 651–652 (1979).
Kirkegaard, K., Buc, H., Spassky, A. & Wang, J. C. Proc. natn. Acad. Sci. U.S.A. 80, 2544–2548 (1983).
Siebenlist, U., Simpson, R. B. & Gilbert, W. Cell 20, 269–281 (1980).
Church, G. & Gilbert, W. Proc. natn. Acad. Sci. U.S.A. 81, 1991–1995 (1984).
Turchinski, M. F., Gus'kova, L. I., Khazai, I., Budovskii, E. I. & Kochetov, M. K. Molec. Biol. (USSR) 4, 343–348 (1969).
Peattie, D. A. Proc. natn. Acad. Sci. U.S.A. 76, 1760–1764 (1979).
Miller, J. A. Experiments in Molecular Genetics (Cold Spring Harbor Laboratory, New York, 1972).
Ish-Horowicz, D. & Burke, J. F. Nucleic Acids Res. 9, 2989–2998 (1981).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Becker, M., Wang, J. Use of light for footprinting DNA in vivo. Nature 309, 682–687 (1984). https://doi.org/10.1038/309682a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/309682a0
This article is cited by
-
Base-resolution UV footprinting by sequencing reveals distinctive damage signatures for DNA-binding proteins
Nature Communications (2023)
-
A whole genome approach to in vivo DNA-protein interactions in E. coli
Nature (1992)
-
Protection against UV-induced pyrimidine dimerization in DNA by triplex formation
Nature (1990)
-
Genomic footprinting with Taq polymerase
Nature (1989)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.