Nature 290, 470 - 474 (09 April 1981); doi:10.1038/290470a0

tRNA punctuation model of RNA processing in human mitochondria

Deanna Ojala, Julio Montoya & Giuseppe Attardi

Division of Biology, California Institute of Technology, Pasadena, California 91125, USA

A 3'-end proximal segment of most of the putative mRNAs encoded in the heavy strand of HeLa cell mtDNA has been partially sequenced and aligned with the DNA sequence. In all cases, the 3'-end nucleotide of the individual mRNA coding sequences has been found to be immediately contiguous to a tRNA gene or another mRNA coding sequence. These and previous results indicate that the heavy (H) strand sequences coding for the rRNA, poly (A)-containing RNA and tRNA species form a continuum extending over almost the entire length of this strand. We propose that the H strand is transcribed into a single polycistronic RNA molecule, which is processed later into mature species by precise endonucleolytic cleavages which occur, in most cases, immediately before and after a tRNA sequence.

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