Letter to the Editor

Cell Research (2014) 24:1274–1277. doi:10.1038/cr.2014.83; published online 1 July 2014

The willow genome and divergent evolution from poplar after the common genome duplication

Xiaogang Dai1,*, Quanjun Hu2,*, Qingle Cai3,*, Kai Feng1,*, Ning Ye1,*, Gerald A Tuskan4,*, Richard Milne5, Yingnan Chen1, Zhibing Wan1, Zefu Wang2, Wenchun Luo2, Kun Wang2, Dongshi Wan2, Mingxiu Wang1, Jun Wang3, Jianquan Liu2 and Tongming Yin1

  1. 1The Southern Modern Forestry Collaborative Innovation Center, Nanjing Forestry University, Nanjing 210037, China
  2. 2State Key Laboratory of Grassland Agro-Ecosystem, College of Life Science, Lanzhou University, Lanzhou 730000, China
  3. 3BGI-Shenzhen, Shenzhen 518083, China
  4. 4Bio-Sciences Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831, USA
  5. 5Institute of Molecular Plant Science, The University of Edinburgh, Daniel Rutherford Building, King's Buildings, Mayfield Road, Edinburgh EH9, 3JH, UK

Correspondence: Tongming Yin, E-mail: tmyin@njfu.edu.cn; Jianquan Liu, E-mail: liujq@lzu.edu.cn; Jun Wang, E-mail: wangj@genomics.org.cn

*These six authors contributed equally to this work.


Dear Editor,

Willows (Salix) and poplars (Populus) are known worldwide as woody species with diverse uses1,2. Although these two genera diverged from each other around the early Eocene3, they share numerous traits, including the same chromosome number of 2n = 38 and the common 'Salicoid' genome duplication with a high macrosynteny4,5. However, most willow species flower early in their lives with short, small and sometimes indistinct stems, and thus differ from poplars in their life histories and habits2. In addition, multiple inter- and intrachromosomal rearrangements have been detected involving chromosomal regions present in both lineages6, suggestive of the likely genomic divergence after the common genome duplication.

In order to test this hypothesis, we sequenced the genome of a shrub willow S. suchowensis, which flowers within two years1,2. Genome sequencing was conducted with a combined approach using Roche/454 and Illumina/HiSeq-2000 sequencing technologies (Supplementary information, Table S1A and S1B), and the statistics of the genome assembly were listed in Supplementary Table S1C. The size of the S. suchowensis genome was estimated to be ~425 Mb and ~429 Mb based on 17-mer analysis and flow cytometry, respectively (Supplementary information, Figure S1A and Table S1D), about 60 Mb smaller than that of P. trichocarpa (approximately 485 ± 10 Mb)4, which is largely consistent with the previous genome measurements of other willow species7. The final length of the assembled sequence was amounted to about 71% (303.8/425 Mb) of the estimated genome size. Sequencing depth distribution showed that ~94% of the assemblies had greater than 20-fold coverage, ensuring a high level of single-base accuracy (Supplementary information, Figure S1B). A detailed analysis found that the ~120 Mb of unassembled genomic sequence consisted mainly of repetitive sequences, and the protein-coding regions were assembled to near completeness. The S. suchowensis genome assembly is of comparable quality to other sequenced plant genomes based on next-generation sequencing (Supplementary information, Table S1E). We further evaluated the assembled genome using two independent EST datasets. The first comprises the EST assemblies from various tissues of the sequenced individual, including tender root, young leaves, bark, non-lignified shoot and vegetative buds. The second consists of EST assemblies from flower buds collected from natural willow stands. These two analyses identified 97.6% and 94.2% of the two EST assemblies in our S. suchowensis genome assembly, separately, with > 95% identity and > 50% coverage of query length, confirming the nearly complete coverage of genic regions (Supplementary information, Table S1F). Core eukaryotic genes identified by CEGMA were further mapped to the predicted gene set, and 97.8% of the NCBI euKaryotic clusters of Orthologous Groups (KOGs) are covered by the predicted willow gene set, confirming the nearly completeness of the genic regions by the present genome assembly (Supplementary information, Table S1G). Assessment of the quality of gene prediction revealed that the overall gene length distributions were similar between S. suchowensis and other plant species with genomes sequenced (Supplementary information, Figure S1C).

In total, we identified 26 599 putative protein-coding genes (Supplementary information, Table S1H and Data S1), 20 261 of which constitute homolgous gene pairs with the P. trichocarpa reference gene set4. Synteny and collinearity analyses indicate that the chromosomal structures are highly similar between the willow and poplar genomes (Figure 1A and 1B), supporting the concept that these two genera share an additional common (Salicoid) whole genome duplication (WGD) event in their evolutionary history4. Estimation with four-fold synonymous third-codon transversion (4DTv) values of the orthologous pairs suggests that the divergence between Salix and Populus took place around 52 million years ago, approximately 6 million years after the additional WGD. The shared WGD was indicated by sharp peaks in 4DTv values of 0.2842 and 0.2052 for S. suchowensis and P. trichocarpa, respectively (Figure 1C). These different 4DTv values might be caused by different evolutionary rates of nucleotide substitution in these two lineages. Using 1 232 single-copy orthologous genes (Supplementary information, Table S1I), we estimated mean substitution rates of 1.09 × 10−9 and 0.67 × 10−9/site/year for S. suchowensis and P. trichocarpa, respectively. This estimated substitution rate is largely consistent with a previous study of other Salix species based on fewer genes6. Our comparisons suggest that S. suchowensis has a significantly higher substitution rate than P. trichocarpa (P < 2.2e-16). However, substitution rates of both species were substantially lower than those of Arabidopsis thaliana and Oryza sativa (Figure 1D). The great differences in evolutionary rates between these two species are highly correlated with their flowering habits: the early-flowering species has faster substitution rates than the long-generation one. This finding is consistent with previous expectations8,9.

Figure 1.
Figure 1 - Unfortunately we are unable to provide accessible alternative text for this. If you require assistance to access this image, please contact help@nature.com or the author

Similarity and divergence between the genomes of Salix suchowensis and Populus trichocarpa. (A) Gene collinearity between S. suchowensis and P. trichocarpa. The x-axis corresponds to the Populus chromosomes; and the y-axis corresponds to the Salix scaffolds. (B) Nucleotide alignments between S. suchowensis scaffolds and 19 P. trichocarpa chromosomes. The 19 poplar chromosomes were scaled in their physical length and displayed in a numeric order. For each chromosome, the black bar on the top represents the Populus chromosome, and the black bar under it represents the Salix scaffolds. (C) 4DTv values were separately calculated with paralogs of S. suchowensis and P. trichocarpa, and with orthologs between S. suchowensis and the other four species, including P. trichocarpa, A. thaliana, V. vinifera and O. sativa. (D) The mean substitution rates were estimated using single-copy genes for S. suchowensis, P. trichocarpa, A. thaliana, V. vinifera and O. sativa.

Full figure and legend (111K)

In order to confirm that the smaller number of genes annotated in S. suchowensis (26 599) than in P. trichocarpa (40 303) was the result of divergent evolution between the two genera after the common genome duplication, we conducted the following analyses. First, the collapsed sequences may lead to the reduced gene number through the fused assembly of homologous genes with multiple copies into a 'single' one. Under this scenario, the collapsed sequences will manifest themselves to those regions of the assembled genome which have elevated read coverage. In order to determine how this artifact might affect our gene annotation, we analyzed the read coverage across the genome. We found a small number of regions with elevated read coverage, covering approximately 38.8 Mb, indicating the presence of collapsed sequences in the assembled genome. Of these, 19.8 Mb were located in the repeat regions, whereas the genic region contained only 308.2 Kb of sequences, which were correlated with just 315 annotated genes. Thus, the collapsed assembly of homologous genes contributed little to the reduced number of genes in the assembled willow genome in the present study.

A second possibility is that Populus retained more gene copies than Salix following genome duplication. To test this, we compared the shrinkage and expansion of gene families within each genome. We extracted the gene family clusters generated by the OrthoMCL pipeline in S. suchowensis and P. trichocarpa. Of the 14 772 gene families shared by the two genomes, 3 434 families contain more genes in poplar than in willow. In contrast, only 627 families contain more genes in willow than in poplar. The remaining 10 711 gene families contain the same number of genes in both species. We found that 1 034 families were unique in P. trichocarpa, while 179 families were specific in S. suchowensis. Comparisons of gene expansions between willow and poplar also suggest that fractions of genes associated with WGD or segmental duplications were higher in poplar than in willow, suggesting that poplar retained more 'Salicoid' duplicates than willow after their divergence (Supplementary information, Table S1J). Even in the families with more genes in willow, these genes were rarely associated with WGD or segmental duplications, but were mainly derived from expansions through lineage-specific tandem duplications and transposons (Supplementary information, Table S1J). These comparisons suggest that willow might have lost more genes than poplar after the common genome duplication.

Both gene variation and genome duplication have contributed greatly to our current species and life diversity10. The genomic divergences between willow and poplar illustrated here highlight their divergent evolution since the common genome duplication, which laid the genetic bases for development of their divergence in life history, habit and other traits2. In addition, willows are promising sources for bioenergy crops due to their high biomass yields1. The availability of this willow genome will help improve productivity and quality of such woody crops for biofuel and other uses.



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This work was supported by the Key Forestry Public Welfare Project (201304102), the National Basic Research Program of China (2012CB114505), the National Natural Science Foundation of China (31125008) and the Program for Innovative Research Team of the Educational Department of China and in Universities of Jiangsu Province. It was also funded by the Doctorate Fellowship Foundation and PAPD (Priority Academic Program Development) program at Nanjing Forestry University and the BioEnergy Science Center, which is a US Department of Energy Bioenergy Research Center supported by the Office of Biological and Environmental Research in the DOE Office of Science. Oak Ridge National Laboratory is managed by UT-Battelle, LLC, under contract DE-AC05-00OR22725 for the Department of Energy.

(Supplementary information is linked to the online version of the paper on the Cell Research website.)

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