FIGURE 4
FROM:
Membrane glycoprotein M6A promotes 
-opioid receptor endocytosis and facilitates receptor sorting into the recycling pathway
Ying-Jian Liang, Dai-Fei Wu, Ralf Stumm, Volker Höllt and Thomas Koch
BACK TO ARTICLEFigure 4.
Effect of M6a on agonist-induced down-regulation of 
-opioid receptors. HEK293 cells stably expressing HA-MOPr with and without co-expression of Myc-M6a were incubated at 37 °C for 16 h in the presence or absence of 5 M DAMGO. The total amount of MOPr was examined by a membrane binding assay, as described in 'Materials and Methods'. The binding of total receptors without DAMGO treatment was set as 100% (Vehicle). Values represent mean 
SEM of 3-5 independent measurements performed in triplicate. *
P<0.05: indicates significant difference between MOPr and MOPr-M6a expressing cells (Student's t-test).
