FIGURE 3
FROM:
MicroRNA let-7b targets important cell cycle molecules in malignant melanoma cells and interferes with anchorage-independent growth
Julia Schultz, Peter Lorenz, Gerd Gross, Saleh Ibrahim and Manfred Kunz
BACK TO ARTICLEFigure 3.
Reporter gene analyses using CCND1 and CCNA 3'UTR constructs. SK-Mel-147 melanoma cells were transfected with the pGL3 Promotor Vector (pGL3), constructs containing the 3'UTR of CCND1 (pGL3-CCND1-3'UTR), or 3'UTR of CCNA (pGL3-CCNA-3'UTR), and co-transfected with either anti-let-7b antagomir or left untransfected (Ø). Single asterisks indicate statistical significance of differences in pGL3-CCND1-3'UTR or pGL3-CCNA-3'UTR luciferase activity compared with pGL3 luciferase activity; double asterisks indicate statistical significance of difference in pGL3-CCND1-3'UTR luciferase activity of anti-let-7b-transfected compared with untransfected cells (P < 0.05; Mann-Whitney-U test).
