FIGURE 4
FROM:
SARS coronavirus entry into host cells through a novel clathrin- and caveolae-independent endocytic pathway
Hongliang Wang, Peng Yang, Kangtai Liu, Feng Guo, Yanli Zhang, Gongyi Zhang and Chengyu Jiang
BACK TO ARTICLEFigure 4.
SARS-CoV entry into cells expressing a dominant-negative mutant of Eps15. Vero E6 cells were transfected with the GFP-tagged construct Eps15 D3
2 (A-C and G-I) or E95/295 (D-F and J-L), 48 h later, cells were incubated with Alexa 594 transferrin (A-F) or infected with spike-bearing pseudovirus (G-L) for 1 h at 37 °C. Expression of Eps15 proteins was monitored by GFP fluorescence. Spike-bearing pseudovirus was detected using anti-spike antibody, followed by incubation with Alexa-568 anti-mouse antibodies.
