FIGURE 3
FROM:
In vitro derivation of functional insulin-producing cells from human embryonic stem cells
Wei Jiang, Yan Shi, Dongxin Zhao, Song Chen, Jun Yong, Jing Zhang, Tingting Qing, Xiaoning Sun, Peng Zhang, Mingxiao Ding, Dongsheng Li and Hongkui Deng
BACK TO ARTICLEFigure 3.
Pancreatic lineage differentiation from human ES cells was further induced with RA. (A) human ES cells cultured in CDM without activin A and RA treatment (bar 100 
m). (B) Small clusters formed from differentiated human ES cells in CDM with activin A and RA treatment (bar 100 m). (C) pdx1 and Amylase staining, ngn3 and C-peptide staining of differentiated human ES cells in CDM after activin A and RA induction. Negative control staining of the differentiated human ES cells was shown in the lower panel (bar 40 m). (D) With both activin A- and RA-induction, the mRNA levels of pdx1, hnf4
and hlxb9 were induced as shown by RT-PCR detection. Without activin A and RA treatment, spontaneously differentiated human ES cells did not express pdx1, hnf4 or hlxb9. The presence of activin A alone induced only weak expression of pdx1 and hnf4 genes and RA alone induced only hlxb9 expression.
