Cell Research

FIGURE 1

FROM:

A highly homozygous and parthenogenetic human embryonic stem cell line derived from a one-pronuclear oocyte following in vitro fertilization procedure

Ge Lin, Qi OuYang, Xiaoying Zhou, Yifan Gu, Ding Yuan, Wen Li, Gang Liu, Tiancheng Liu and Guangxiu Lu

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Figure 1.

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Isolation and characterization of chHES-32 cells with commonly used markers. (A) The one-pronuclear oocyte used for deriving the chHES-32 cells. (B) The original collapsed blastocyst on d5. (C) The undifferentiated chHES-32 cell colonies growing on mitotically inactivated human feeder layers; the inset is a higher magnified image of one colony. (D–I) Expression of molecular markers in undifferentiated chHES-32 cells. Undifferentiated cells stain positive for AKP (D), SSEA-3 (E), SSEA-4 (F), TRA1-60 (G), TRA1-81 (H) and OCT-4 (I).

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