FIGURE 2
FROM:
Skeletal myogenesis by human embryonic stem cells
Jun Ke Zheng, Yi Wang, Aditi Karandikar, Qian Wang, Hui Gai, Ai Lian Liu, Chao Peng and Hui Zhen Sheng
BACK TO ARTICLEFigure 2.
Myogenic differentiation in culture. Before 5-azacytidine treatment (left panels in each pairs), most cells expressed c-Met (green), while a relatively small population in the culture expressed myo D and Pax3 (red nuclei, arrows). Pax7 (red nuclei, arrow) was not expressed. After treatment with 5-azacytidine (right panels), c-Met expression reduced significantly and Pax3 reduced slightly. MyoD and Pax7 expression increased. Following immunofluorescent staining, cells were counterstained with Hoechst 33342 (blue). Both the H1 and SH39 lines were tested for myogenic differentiation in culture. Scale bars = 25 
m.