Cell Research

FIGURE 2

FROM:

Molecular and phenotypic characterization of human amniotic fluid cells and their differentiation potential

Patrizia Bossolasco, Tiziana Montemurro, Lidia Cova, Stefano Zangrossi, Cinzia Calzarossa, Simona Buiatiotis, Davide Soligo, Silvano Bosari, Vincenzo Silani, Giorgio Lambertenghi Deliliers, Paolo Rebulla and Lorenza Lazzari

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Figure 2.

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(A) Specific osteogenic (osteocalcin), adipogenic (PPARgamma2) and some embryonic stem cell markers (Oct-4, Rex-1, Runx-1) were present pre-treatment in our samples. No muscle expression (MyoD, Myogenin, MRF4, Desmin and Myf-5) was detected both before and after specific induction. Amnio Pre: Lane 1: GAPDH. Lane 2: MyoD. Lane 3: Myogenin. Lane 4: MRF4. Lane 5: Desmin. Lane 6: Myf-5. Lane 7: PPARgamma2. Lane 8: Osteocalcin. Lane 9: Oct-4. Lane 10: ABCG2. Lane 11: Runx-1. Lane 12: Rex-1. Lane 13: hTERT. Lane 14: SOX-2. Lane 15: FGF-4. Lane 16: PDX-1.Amnio Post: Lane 1: GAPDH. Lane 2: MyoD. Lane 3: Myogenin. Lane 4: MRF4. Lane 5: Desmin. Lane 6: Myf-5. Lane 7: PPARgamma2. Lane 8: Osteocalcin. (B) the neural markers NSE, MAP2, Nestin and GAP43 showed no differences both pre- and post-treatment by RT-PCR. (C) a semiquantitative RT-PCR showed an increase of beta tubulin III and GFAP expression post-treatment induction. (D) Western blot analysis revealed the presence of GFAP neural specific isoform (50plusminus1 kDa). As a control we used neural stem cell derived protein extract showing the presence of both the isoforms (40plusminus1 and 50plusminus1 kDa).

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