Article
Cell Research (2005) 15, 207–211. doi:10.1038/sj.cr.7290288
Identification of connexin 50 and 57 mRNA in A-type horizontal cells of the rabbit retina
He HUANG1,*, Hui LI1,* and Shi Gang HE1
1Institute of Neuroscience, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 Yueyang Road, Shanghai 200031, China
Correspondence: He HUANG, Tel: +86-21-5492 1771; Fax: +86-21-5492 1735; E-mail: huanghe@sibs.ac.cn
*These authors contributed equally to the work.
Received 15 October 2004; Revised 10 January 2005; Accepted 17 January 2005.
Abstract
Horizontal cells (HCs) mediate negative feedback to photoreceptors. In the mammalian retina, there are two types of HCs, which are extensively coupled to neighboring cells through homologous gap junctions. The permeability and therefore the strength of feedback can be regulated by light intensity, dopamine and many other factors. However, the component(s) of the most prominent gap junctions, those between A-type HCs in the rabbit retina, is still unknown. In this study, we compared the sequences of many types of mammalian connexins, obtained partial sequences of rabbit connexin 50 and 57. Using specific primers designed against the rabbit sequences, we identified mRNAs of connexin 50 and/or 57 in visually selected single A-type HC using multiplex RT-PCR.
Keywords:
gap junctions, dissociated retinal neurons, single cell multiplex RT-PCR, sequencing
MORE ARTICLES LIKE THIS
These links to content published by NPG are automatically generated.
RESEARCH
Separate blue and green cone networks in the mammalian retinaNature Neuroscience Article (01 Jul 2004)
Ischemia-Induced Brain Damage Depends on Specific Gap-Junctional CouplingJournal of Cerebral Blood Flow & Metabolism Original Article
Ischemia-Induced Brain Damage Depends on Specific Gap-Junctional CouplingJournal of Cerebral Blood Flow & Metabolism Original Article
