Short Communication
Cell Research (2003) 13, 509–514. doi:10.1038/sj.cr.7290195
Gene expression profile favoring phenotypic reversion: a clue for mechanism of tumor suppression by NF-IL6 3'UTR
Ding Gan LIU1, Qiu Hong JIANG1, Yun Yi WEI1, Li SUN1, Bei Bei FU1, Fu Kun ZHAO2 and Qiong ZHOU1
- 1State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China. E-mail: dgliu@sibs.ac.cn
- 2Key Laboratory of Proteomics, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China. E-mail: dgliu@sibs.ac.cn
Correspondence: Dr. Ding Gan LIU, Tel: 021-54921135 E-mail: dgliu@sibs.ac.cn
Received 6 September 2003; Revised 27 November 2003; Accepted 29 November 2003.
Abstract
Transfection of cDNA in 3'untranslated region of human nuclear factor for interleukin-6 (NF-IL6 3'UTR) induced tumor suppression in a human hepatoma cell line. cDNA array analysis was used to reveal changes in gene expression profile leading to tumor suppression The results indicate that this suppression was not due to activation of dsRNA-dependent protein kinase, nor to inactivation of oncogenes; rather, all the changes in expression of known genes, induced by NF-IL6 3'UTR cDNA may be ascribed to the suppression of cellular malignancy. Therefore, our results imply that this 3'untranslated region may have played role of a regulator of gene expression profile.
Keywords:
cDNA array, NF-IL6, hepatoma, 3'UTR, tumor suppression
Abbreviations:
NF-IL6, nuclear factor for interleukin-6 expression; PKR, double-stranded RNA-dependent protein kinase; 3'UTR, 3'untranslated region
