Cell Research

FIGURE 1

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The involvement of p38 MAPK in transforming growth factor bold italic beta1-induced apoptosis in murine hepatocytes

Jin Hui LIAO, Jun Song CHEN, Min Qiang CHAI, Sheng ZHAO and Jian Guo SONG

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Figure 1.

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The inhibition of TGF-beta1-induced apoptosis by SB202190 (SB) AML12 cells were treated with TGF-beta1 (10 mug/ml) in the presence or absence of SB 202190 (10 muM). Cells were pre-incubated with SB202190 for 30 min before the addition of TGF-beta1. Cells were further incubated for 24 h, apoptosis was then determined. (A)Cells were stained by acridine orange and ethidium bromide. Fluorescence was visualized immediately with a fluorescent microscope. (B) DNA fragmentation was detected as described in "Materials and Methods".

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