Regular Article
Cell Research (2000) 10, 71–77. doi:10.1038/sj.cr.7290037
Using a non-radioisotopic, quantitative TRAP-based method detecting telomerase activities in human hepatoma cells
Ru Gang ZHANG1, Xing Wang WANG1, Jin Hui YUAN1, Li Xia GUO1 and Hong XIE1
1Shanghai Institute of Cell Biology, Chinese Academy of Sciences, Shanghai 200031, China
Correspondence: Hong XIE, E-mail: xiehong@sunm.shcnc.ac.cn
Received 9 December 1999; Revised 18 January 2000; Accepted 21 January 2000.
Abstract
A non-radioisotopic, quantitative TRAP-based telomerase activity assay was established mainly by using SYBR Green-I staining instead of radioisotope. Comparing with conventional radioisotope based method, it was better in reproducibility and accuracy. Using this method, we found telomerase activities were absent in normal human liver cells, while detected in all of four human hepatoma cell lines (BEL-7404, SMMC-7721, QGY-7903 and HCCM) without significant differences.
Keywords:
Telomerase, non-radioisotopic telomerase assay, human liver cells, human hepatoma cells
