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Response of BALB/c mice to a monovalent influenza A (H1N1) 2009 split vaccine

Cellular & Molecular Immunology volume 7, pages 116–122 (2010)Cite this article

Abstract

The novel influenza A (H1N1) 2009 virus has emerged to cause the first pandemic of the twenty-first century. Disease outbreaks caused by the influenza A (H1N1) virus have prompted concerns about the potential for a pandemic and have driven the development of vaccines against this subtype of influenza A. In this study, we developed a monovalent influenza A (H1N1) split vaccine and evaluated its effects in BALB/c mice. Mice were immunized subcutaneously with 2 doses of the vaccine containing hemagglutinin (HA) alone or HA plus an aluminum hydroxide (Al(OH)3) adjuvant. Immunization with varying doses of HA (3.75, 7.5, 15, 30, 45 or 60 µg) was performed to induce the production of neutralizing antibodies. The vaccine elicited strong hemagglutination inhibition (HI) and microneutralization, and addition of the adjuvant augmented the antibody response. A preliminary safety evaluation showed that the vaccine was not toxic at large doses (0.5 ml containing 60 µg HA+600 µg Al(OH)3 or 60 µg HA). Moreover, the vaccine was found to be safe at a dose of 120 µg HA+1200 µg Al(OH)3 or 120 µg HA in 1.0 ml in rats. In conclusion, the present study provides support for the clinical evaluation of influenza A (H1N1) vaccination as a public health intervention to mitigate a possible pandemic. Additionally, our findings support the further evaluation of the vaccine used in this study in primates or humans.

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Acknowledgements

We thank Hualan Vaccine Inc. (Xinxiang, Henan, China) for providing the influenza A (H1N1) split vaccine. We are grateful to Dr H. Fen-Tian for his critical reading of the manuscript. This work was carried out in part with funding from the National Scientific and Technical Supporting Program of China (2009CB522102) and National Programs for High Technology Research and Development of China (2006AA02Z450). No other potential conflict of interest relevant to this article exists.

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  1. Beijing Institute of Microbiology and Epidemiology, State Key Laboratory of Pathogen and Biosecurity, Beijing, China

    Penghui Yang, Li Xing, Chong Tang, Weihong Jia, Zhongpeng Zhao, Kun Liu, Xiao Gao & Xiliang Wang

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Correspondence to Xiliang Wang.

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Yang, P., Xing, L., Tang, C. et al. Response of BALB/c mice to a monovalent influenza A (H1N1) 2009 split vaccine. Cell Mol Immunol 7, 116–122 (2010). https://doi.org/10.1038/cmi.2009.116

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