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Adenovirus replication–competent vectors (KD1, KD3) complement the cytotoxicity and transgene expression from replication-defective vectors (Ad-GFP, Ad-Luc)

Cancer Gene Therapy volume 9, pages 651–654 (2002)Cite this article

Abstract

The successful clinical application of adenovirus (Ad) in cancer control has been of limited success because of the current inability to infect the majority of cancer cells with a large amount of vector. In this study, we show that when human lung tumors growing in immunodeficient nude mice were coinfected with a replication-defective (RD) Ad vector expressing green fluorescent protein and a replication-competent (RC) Ad vector named KD3, KD3 enhanced the expression of green fluorescent protein throughout the tumor. Also, KD3 and another RC vector named KD1 complemented the expression of luciferase from a RD vector in a human liver tumor xenotransplant in nude mice. Altogether, these results suggest that the combination of a RD vector with a RC vector might be a more effective treatment for cancer than either vector alone due to more widespread dissemination of the virus.

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Acknowledgements

We are grateful to Jeffery Whitsett who provided Ad-Luc, and Ratna Ray who provided Ad-GFP. A portion of this research was supported by National Institutes of Health Grants CA71704 and CA81829 (to WSMW).

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Authors and Affiliations

  1. Department of Surgery, Faculty of Medicine, Imperial College, Hammersmith Hospital Campus, London, UK

    Nagy A Habib & Ragai Mitry

  2. Human Research Institute, Des Moines, Iowa, USA

    Prem Seth

  3. Department of Molecular Microbiology and Immunology, Saint Louis University School of Medicine, St. Louis, Missouri, USA

    Mohan Kuppuswamy, Konstantin Doronin, Karoly Toth, Peter Krajcsi, Ann E Tollefson & William S M Wold

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  1. Nagy A Habib
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  2. Ragai Mitry
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Correspondence to Nagy A Habib.

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Habib, N., Mitry, R., Seth, P. et al. Adenovirus replication–competent vectors (KD1, KD3) complement the cytotoxicity and transgene expression from replication-defective vectors (Ad-GFP, Ad-Luc). Cancer Gene Ther 9, 651–654 (2002). https://doi.org/10.1038/sj.cgt.7700481

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  • DOI: https://doi.org/10.1038/sj.cgt.7700481

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