Cancer Gene Therapy

FIGURE 6

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Adenovirus E1a protein enhances the cytotoxic effects of the herpes thymidine kinase-ganciclovir system

Carlos Parada, Javier Hernández Losa, Juan Guinea, Víctor Sánchez-Arévalo, Víctor Fernández Soria, Luis Alvarez-Vallina, Ricardo Sánchez-Prieto and Santiago Ramón y Cajal

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Figure 6.

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Cytological features of GCV-treated cells. After treatment with GCV, E1a-TK-expressing cells showed a striking enlargement of the nuclei and cytoplasm. Note that at the same magnification (times100), the E1a-TK cells treated with GCV show large cytoplasms compared to the same cells without GCV. Crystal violet staining. a: NIH-3T3 E1a-TK cells without GCV. b: NIH-3T3 E1a-TK cells after 3 days of treatment with GCV. c: MDA-MB435 E1a-TK cells without GCV. d: MDA-MB435 E1a-TK cells with GCV.

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