Cancer Gene Therapy

FIGURE 5

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Selective suppression of cathepsin L by antisense cDNA impairs human brain tumor cell invasion in vitro and promotes apoptosis

Natas carona Levic caronar, Ricardo A Dewey, Emma Daley, Timothy E Bates, Derek Davies, Janko Kos, Geoffrey J Pilkington and Tamara T Lah

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Figure 5.

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Apoptosis of human glioblastoma cell lines induced by STS. IPTP, IPTPvgfp, IPTP4asn, and IPTP24sn cells were treated with 1 muM STS and the relative rate of apoptosis was determined after 24 hours, using acridine orange/ethidium bromide staining. IPTP4asn cells had significantly higher rate of apoptosis (54% of cells), whereas IPTP24sn cells were more resistant to apoptosis (13% of cells). Bars represent mean value of three independent experiments and standard error of the mean (meanplusminusSEM). ***Indicates statistically significant (P<.05) difference compared with the parental IPTP cell line.

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