FIGURE 2
FROM:
Selective suppression of cathepsin L by antisense cDNA impairs human brain tumor cell invasion in vitro and promotes apoptosis
Nata
a Levi
ar, Ricardo A Dewey, Emma Daley, Timothy E Bates, Derek Davies, Janko Kos, Geoffrey J Pilkington and Tamara T Lah
Figure 2.
Cathepsin L-type activity in cell lysates of selected clones of IPTP cells. Cathepsin L-type activity was measured using the fluorogenic substrate Z-Phe-Arg-AMC. The mean values of specific enzymatic activities in IPTP, IPTPvgfp, IPTP4asn, and IPTP24sn cells were compared. Cathepsin L-type activity was reduced by 50% in IPTP4asn and significantly elevated by 143% in IPTP24sn cells compared with the parental cells. Bars represent mean value of three independent experiments and standard error of the mean (mean
SEM). ***Indicates a statistically significant (P<.05) difference compared with the parental IPTP cell line.
