Cancer Gene Therapy

FIGURE 3

FROM:

Adenovirus-mediated PTEN treatment combined with caffeine produces a synergistic therapeutic effect in colorectal cancer cells

Yuji Saito, Began Gopalan, Abner M Mhashilkar, Jack A Roth, Sunil Chada, Louis Zumstein and Rajagopal Ramesh

BACK TO ARTICLE

Figure 3.

Figure and tables index
Unfortunately we are unable to provide accessible alternative text for this. If you require assistance to access this image, please contact help@nature.com or the author

Induction and abrogation of G2/M cell-cycle arrest due to overexpression of PTEN and treatment with caffeine. (a) HCT116dotp53 (+ / +) colorectal cancer cells and (b) CCD-18Co normal colorectal fibroblast cells were treated with PBS, Ad-Luc, Ad-PTEN, caffeine, combinations of caffeine with Ad-Luc or Ad-PTEN, or 20 muM LY294002. Cells were harvested 72 hours after treatment and cell-cycle analysis was performed by using flow cytometry. In total 20,000 events were captured for each treatment, and the data are shown as histograms. The cell-cycle phase is represented on the X-axis. Data were generated in duplicate; the average values are shown. Bars denote standard error (SE).

Figure and tables index
BACK TO ARTICLE