Cancer Gene Therapy

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Adenovirus-mediated PTEN treatment combined with caffeine produces a synergistic therapeutic effect in colorectal cancer cells

Yuji Saito, Began Gopalan, Abner M Mhashilkar, Jack A Roth, Sunil Chada, Louis Zumstein and Rajagopal Ramesh

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Figure 2.

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Induction of apoptosis by treatment with combinations of Ad-PTEN and caffeine. (a) The numbers of cells at phase sub-G0/G1 (apoptotic cells) in colorectal cancer cells HCT116dotp53 (+ / +) and normal colorectal fibroblast cells CCD-18Co were analyzed by flow cytometry 72 hours after treatment with PBS, Ad-Luc, Ad-PTEN, caffeine, or combinations of caffeine with Ad-Luc or Ad-PTEN. Data represent the means of two experiments. Error bars denote standard error (SE). (b) Apoptotic analysis of HCT116dotp53 (+ / +) cancer cells and CCD-18Co normal cells by Hoechst 33258 staining was performed 72 hours after treatment with PBS, caffeine, or combinations of Ad-PTEN and caffeine. Treatment with combinations of Ad-PTEN and caffeine induced apoptosis in cancer cells but not in normal cells. Arrows indicate apoptotic cells (magnification times 400).

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