Review

Subject Category: Experimental Medicine

Citation: Cell Death and Disease (2011) 2, e213; doi:10.1038/cddis.2011.96
Published online 6 October 2011

Hydrogen peroxide: a Jekyll and Hyde signalling molecule

D R Gough1 and T G Cotter1

1Tumour Biology Laboratory, Biochemistry Department, Bioscience Research Institute, University College Cork, Cork, Ireland

Correspondence: TG Cotter, Tumour Biology Laboratory, Biochemistry Department, Bioscience Research Institute, University College Cork, Cork 021, Ireland. Tel: +353 21 490 1321; Fax: +353 21 490 1382; E-mail: t.cotter@ucc.ie

Received 30 June 2011; Revised 31 August 2011; Accepted 31 August 2011

Edited by A Finazzi-Agró

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Abstract

Reactive oxygen species (ROS) are a group of molecules produced in the cell through metabolism of oxygen. Endogenous ROS such as hydrogen peroxide (H2O2) have long been recognised as destructive molecules. The well-established roles they have in the phagosome and genomic instability has led to the characterisation of these molecules as non-specific agents of destruction. Interestingly, there is a growing body of literature suggesting a less sinister role for this Jekyll and Hyde molecule. It is now evident that at lower physiological levels, H2O2 can act as a classical intracellular signalling molecule regulating kinase-driven pathways. The newly discovered biological functions attributed to ROS include proliferation, migration, anoikis, survival and autophagy. Furthermore, recent advances in detection and quantification of ROS-family members have revealed that the diverse functions of ROS can be determined by the subcellular source, location and duration of these molecules within the cell. In light of this confounding paradox, we will examine the factors and circumstances that determine whether H2O2 acts in a pro-survival or deleterious manner.

Keywords:

NADPH oxidase; reactive oxygen species; redox signalling

Abbreviations:

ROS, reactive oxygen species; H2O2, hydrogen peroxide; O2·, superoxide; OH, hydroxyl radical; Nox, NADPH oxidase; FAD, flavin adenine dinucleotide; DCFH2DA, dichlorodihyrofluorescein diacetate; DHE, dihydroethidium; DHR, dihydrorhodamine; GFP, green fluorescent protein; roGFP, reduction–oxidation-sensitive green fluorescent protein; HyPER, hydrogen peroxide sensor; Prx1, peroxiredoxin-1; NO, nitric oxide; NOS, nitric oxide synthase; RNS, reactive nitrogen species; ONOO, peroxynitrite; NO2, nitrogen dioxide; PTK, protein tyrosine kinase; PTP, protein tyrosine phosphatase; DSP, dual-specificity phosphatase; ERK, extracellular signal-regulated kinase; EPO, erythropoietin; PTEN, phosphatase and tensin homologue; PIP, phosphatidylinositol triphosphate; TNF, tumour necrosis factor; TGF, transforming growth factor; HIF, hypoxia-inducible factor; AP-1, activator protein-1; PDGF, platelet-derived growth factor; IFN, interferon; LPS, lipopolysaccharide; STAT, signal transducer and activator of transcription; PKC, protein kinase-C; VSMC, vascular smooth muscle cell; PMA, phorbol-12-myristate-13-acetate; EGFR, epidermal growth factor receptor; WRN, Werner protein gene; BLT2, leukotriene-B4 receptor-2; ECM, extracellular matrix; ANGPTL4, angiopoietin-related protein-4

Reactive oxygen species (ROS) are a family of molecules that include highly reactive free oxygen radicals (e.g., superoxide anion [O2·] and the hydroxyl radical [OH]) and the stable ‘diffusable’ non-radical oxidants (e.g., hydrogen peroxide [H2O2]). H2O2 and superoxide have been the main investigative foci of ROS biology in recent years, and given the fact that superoxide is rapidly converted to H2O2 in the cell, we will concentrate on H2O2 as the principal ROS member. H2O2 was first isolated by Thénard in 1818 and it was quickly recognised that high concentrations of H2O2 result in cell injury by damaging key cellular molecules such as DNA and lipids.1 Later, elevated levels of ROS became synonymous with genomic instability prompting cell death or carcinogenesis.2

Commoner et al.3 first described biological ROS production in 1954. Nearly a decade later, landmark discoveries occurred, identifying two major sources of such ROS, mitochondrial superoxide generation and the phagocytic respiratory burst. The first record of mitochondrial superoxide generation was reported by Boveris et al.4 During oxidative phosphorylation and transfer of energy along the electron transport chain, 1–5% of electrons escape from complexes I and III, forming superoxide prematurely in the presence of oxygen.5 This process of superoxide generation, termed electron leak, has been implicated in the pathophysiology of numerous diseases.6 Babior, in the 1960s, was among the first to suggest a role for ROS in innate immunity. He hypothesised that the elevated leukocyte oxygen consumption and the concurrent increase in cellular O2· accompanying bacterial phagocytosis was vital to the bactericidal activity of granulocytes.7 The rapid increase in the redox status of neutrophils and macrophages while engulfing pathogens became known as the ‘respiratory burst’. The enzyme responsible was named gp91phox, now known as NADPH oxidase-2 (Nox2).8

In the 1970s, a small collection of studies reported that exogenously added H2O2 could mimic the signalling activity of insulin.9 This revelation remained within small free radical research circles until publications in the 1980s reported that exogenous H2O2 could also stimulate cell proliferation at lower concentrations.10 Subsequent research into the ROS-generating Nox enzymes revealed the true physiological roles of endogenous H2O2.11 Interest in the Nox family intensified in the 90s and 00s. Gene profiling of gp91phox; identification of seven Nox isoforms (Nox1–5 and Duox1 and 2) and subunits (p67phox, p47phox, p40phox, and Rac1/2); and prolific expression of these enzymes in non-phagocytic cell types became clear through the work of David Lambeth12 and others. It was demonstrated that Nox enzymes have a fundamental role in numerous physiological processes, including survival signalling.

Pivotal to this expanding field of research was elucidating the precise mechanism through which H2O2 and other ROS could modulate signalling pathways. The widely accepted hypothesis proposed by the Tonks group describes reversible inhibition of phosphatases that negatively regulate signalling cascades through oxidation of redox-sensitive cysteine residues.13 We now know that ROS have opposing roles in the cell under specific conditions. This review will examine factors such as source and site of H2O2 formation to further discuss this conflicting role of cellular ROS.

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Cellular ROS Generation

Nox enzymes, activated by various growth factors and cytokines, generate H2O2 or O2· for signalling processes such as proliferation, migration and survival.14 Several other sources of endogenous ROS exist such as mitochondria, xanthine oxidase, lipoxygenase and myeloperoxidase. Notably, they fail to show the diverse physiological functions attributed to the Nox family, and hence, will not be discussed further.

The original hypothesis of Nox-mediated ROS generation is based on the gp91phox/Nox2 prototype characterising Nox proteins as integral membrane proteins (Figure 1). Six transmembrane domains form a channel to allow successive transfer of electrons. Electrons are transferred from NADPH (converting it to NADP) to flavin adenine dinucleotide (FAD) to haeme and finally to oxygen to form superoxide.12 Neutrophil stimulation leads to the assembly of an active Nox2 complex at the plasma membrane. This complex comprises a catalytic subunit, the integral membrane protein gp91phox and a p22phox subunit. Activation of this catalytic core relies on the recruitment of several cytosolic protein subunits.15 This complex then releases O2· at micromolar concentrations into the phagosome, killing the pathogen, a process known as the respiratory burst.16 By contrast, little is known about the assembly/activation of the other Nox-family members. Cytokines and growth factors induce ROS production through activation of locally recruited Noxs in non-phagocytic cell types.14 Nox1, 2, 3 and 5 produce O2·, whereas Duox1 and 2, and more recently Nox4, are known to produce H2O2 directly because to a peroxidase-like domain at their N-terminus.17, 18 The consequence of differential production of ROS members by Nox enzymes has yet to be established.

Figure 1.
Figure 1 - Unfortunately we are unable to provide accessible alternative text for this. If you require assistance to access this image, please contact help@nature.com or the author

Nox2 structure. Exposure to pathogenic organism triggers Nox2 complex assembly in the neutrophil by recruiting various subunits to the plasma membrane. The activated Nox complex then releases superoxide in micromolar concentrations into the phagosome, thus killing the pathogen

Full figure and legend (151K)

Accurate quantification and localisation of H2O2 have been the rate-limiting factors in ROS cell signalling research. This stumbling block is further compounded by recent evidence suggesting that H2O2 is not as freely diffusible as once thought. Miller et al.19 demonstrated that aquaporin-3 regulated the transmembrane movement of Nox-derived H2O2. The most prevalent method of imaging biological ROS is through use of fluorescent redox-sensitive dyes such as 2′,7′-dichlorofluorescein diacetate or similar (dihydroethidium, dihydrorhodamine, MitoSox Red and Amplex Red) (Figure 2). Widely acknowledged reservations exist regarding specificity, cell/tissue uptake and subcellular diffusion dynamics of fluorescent dyes that demand cautious interpretation.20 Fluorescent probes such as green fluorescent protein (GFP) and variants (e.g., reduction–oxidation-sensitive GFP (roGFP) and hydrogen peroxide sensor (HyPER)) incorporate redox-sensitive cysteines, becoming fluorescent in the presence of particular ROS.21 The advantages of fluorescent probes include greater sensitivity and specificity, signal reversibility and easy modification with targeting sequences to allow subcellular expression, for example, endoplasmic reticulum.22 Further developments include generation of transgenic animals expressing these redox-sensitive proteins, thus creating invaluable in vivo models, for example, zebrafish.23 Several cell-free assays have been used to measure O2· and H2O2 production as a reflection of Nox activity, yet these methods shed little light on H2O2 as a second messenger molecule in subcellular domains.24

Figure 2.
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Subcellular localisation of Nox-generated H2O2. Multi-photon microscopy images of Nox-generated ROS in MV411 acute myeloid leukaemia cells (courtesy of Dr Lavinia Bhatt). The ROS-sensitive dye, Peroxy Orange-1 (PO1), stains red in the presence of ROS. The fluorescence observed shows a staining pattern consistent with the endoplasmic reticulum

Full figure and legend (34K)

We now understand that non-phagocytic Nox enzymes are no longer confined to the plasma membrane. They have been identified in numerous subcellular compartments such as the endoplasmic reticulum, nucleus and mitochondria.22, 25, 26 The seven Nox isoforms show a complex heterogeneity in which one or more isoforms may be located in several subcellular compartments within a single cell type.27 This expression profile is echoed by the varied subcellular expression levels of both antioxidant enzymes and redox-sensitive protein targets.28 Indeed expression of Nox enzymes in specific subcellular locations can dictate participation in distinct signalling pathways. Ushio-Fukai29 published a thorough review on the compartmentalisation of Noxs affecting immune signalling pathways. Several papers describing compartmentalised H2O2 survival signalling have emerged recently.30, 31, 32

A complex array of enzymatic (i.e., superoxide dismutase, catalase, thioredoxin and peroxiredoxin (Prx)) and non-enzymatic (i.e., glutathione; flavonoids; and vitamins A, C and E) antioxidant systems protect cells against ROS damage (Table 1). Effective antioxidant activity is necessary to buffer fluctuations in the cellular redox status and avoid irreversible oxidation of integral cellular macromolecules such as proteins, lipids and DNA. Recent data have shown that certain antioxidant members are precisely regulated by Nox-driven signalling to channel H2O2 to colocalised target proteins. Toledano et al.33 examine reversible inactivation through phosphorylation of Prx1 colocalised to Nox, c-Src and receptor tyrosine kinases. In consequence to growth factor and cytokine stimulation, kinase-driven pathways are thought to both activate Nox activity and moreover, phosphorylate and thus inactivate local Prx1. This process ensures direct, efficient H2O2 delivery to the target protein.


The intimate relationship that exists between nitric oxide (NO·), another second messenger molecule, and the ROS family warrants mention. NO synthases (NOSs) metabolise arginine to citrulline and generate freely diffusible NO·, a small diatomic molecule with one unpaired electron. At lower concentrations, NO· reacts with certain target proteins mainly through post-translational S-nitrosylation, thus regulating cell survival, smooth muscle tone and immune signalling.34 Higher concentrations of NO· often trigger ‘indirect reactions’ requiring an intermediary reaction with oxygen or O2 to produce reactive nitrogen species (RNS). This well-recognised crosstalk with ROS members is known to generate a spectrum of harmful oxidants comparable to excessive ROS, including peroxynitrite (ONOO) and nitrogen dioxide (NO2). This strangely familiar dichotomy, mirroring ROS biology, is governed by similar parameters, including subcellular location and concentration. The double-edged sword nature of NO reactions is exemplified in the setting of apoptosis. Overproduction of RNS (nitrosative stress), as with oxidative stress, can potentially trigger cell death processes such as DNA fragmentation and lipid oxidation.35 However, RNS can also have a protective role involving nitrosation of caspases and PARP, leading to inhibition of apoptosis.36

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Redox Protein Targets

Understanding the mechanism through which H2O2 modulates signalling pathways is paramount to unearthing the signalling role of Noxs. In keeping with Nox activity, finely controlled, modest fluctuations of the cellular redox status have been shown to be capable of reversible modulation of signalling cascades. Three principal mechanisms of survival pathway activation have been proposed: (a) inhibition of phosphatases, (b) activation of tyrosine kinases and (c) transcription factor activation (Figure 3).

Figure 3.
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Nox regulation of the PI3K/AKT pathway. Activation of Nox activity occurs upon growth factor stimulation. This happens through recruitment of various protein subunits or by induction of Nox isoform expression. Nox-derived H2O2 then regulates kinase-driven survival signalling, for example, the PI3K/AKT pathway, by three major mechanisms. The cysteine residues located at the active sites of specific phosphatases are susceptible to reversible oxidation. This oxidation results in the inhibition of these phosphatases that negatively regulate survival signalling, thus propagating a pro-survival effect. Nox-generated ROS can also stimulate many pro-survival kinases (Src) and transcription factors (NF-kB), resulting in enhanced survival signalling

Full figure and legend (172K)

A large body of evidence identifies cysteine residues as the most likely targets of Nox-generated ROS. Reversible oxidation of cysteine residues occurs when sulphenic acid intermediates (Cys-SOH) are formed. Reversal of this reaction is mediated by incubation with thiol compounds. Interestingly, this process of protein reduction is thought to be equally significant as Nox enzymes in the redox regulation of signalling pathways.37 Importantly, exposure of the highly conserved cysteine residue to excessive ROS leads to irreversible oxidation of the cysteine residue to sulphinic and sulphonic acid, a process that often accompanies cell death/injury processes.38, 39 Several signalling pathways are driven by protein tyrosine kinases (PTKs) through phosphorylation.40 By contrast, phosphatases dephosphorylate signalling proteins, resulting in their inactivation. Phosphatases, a structurally diverse family of receptor-like non-transmembrane enzymes, target specific substrates in vivo and are critical regulators of signalling pathways. The phosphatase super-family includes protein tyrosine phosphatases (PTPs), dual-specificity phosphatases (DSPs) and serine/threonine phosphatases.14

PTPs, including PTP-1B, SHP1 and SHP2, represent the most well-described in vivo targets of Nox-mediated H2O2 signalling. The PTP signature motif, HC(X)5R(S/T), creates a unique environment for the catalytic cysteine residue. The presence of a conserved arginine residue confers an unusually low pKa, hence rendering the cysteine residue highly susceptible to oxidation.13 Oxidation of the cysteine residue results in inhibition of activity because the modified cysteine can no longer function as a phosphate acceptor. Colocalisation of Nox4 and PTP-1B at the endoplasmic reticulum results in enhanced extracellular signal-regulated (ERK) signalling and proliferation upon reversible cysteine oxidation.22 Nox2 activity has been shown to inactivate SHP2 phosphatases thus enhancing erythropoietin (EPO)-induced STAT5 (signal transducer and activator of transcription-5) signalling.41 Oxidative inhibition of DSPs such as phosphatase and tensin homologue (PTEN) and cdc25 results in the formation of a disulphide bond between oxidised cysteines inactivating phosphatase activity and furthermore preventing irreversible oxidation.42 Oxidative inhibition, for example by Nox1, prevents PTEN-mediated dephosphorylation of lipid phosphatidylinositol triphosphate-3 (PIP3) to PIP2, allowing recruitment of PH-containing proteins to the plasma membrane, thus augmenting PI3K/AKT survival signalling.43 The Ser/Thr phosphatases (PP1, PP2A, PP2B and PP2C) dephosphorylate serine and threonine, which are the main phosphorylation sites in the transduction of the kinase-driven PI3K/AKT survival pathway. We demonstrated that BCR-ABL-induced Nox4 expression led to attenuation of PP2A activity and consequent upregulation of PI3K/AKT signalling.44

Evidence also exists for ROS-mediated activation of PTKs such as Src. For example, upon cell attachment to the extracellular matrix (ECM) and associated generation of H2O2, the tyrosine kinase Src becomes oxidised at two cysteine residues and thus becomes activated.45 Moreover, antioxidant treatment of cells that express an oncogenic form of Src (v-Src), or mutation of v-Src cysteine residues, reduces the potency of v-Src to transform cells.46 ROS induction of gene expression has been extensively investigated. ROS-mediated expression of tumour necrosis factor-α (TNFα), transforming growth factor-β1 (TGF-β1), angiotensin and others occurs by two distinct pathways, either downstream from signalling pathways described earlier (e.g., MAPK) or through redox-sensitive transcription factors, (e.g., NF-kB, hypoxia-inducible factor-1α (HIF-1α), p53 and activator protein-1 (AP-1)).14 These transcription factors contain cysteine residues in their DNA-binding domains thus rendering them susceptible to oxidative modulation. It has been shown that Src-mediated activation of HIF-1α occurs through Nox-generated ROS and not by direct phosphorylation as previous thought.47

H2O2 can modulate enzyme activity by several differing mechanisms (Figure 3). Identification of specific protein targets of Nox-mediated ROS is vital to delineating their ever-expanding roles in cellular signalling pathways.

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Growth Factor-Induced ROS Generation

Nox activity increases upon stimulation of the relevant receptors and regulates many downstream survival signalling pathways, including PI3K/AKT and MAPK.48 Signalling mechanisms coupling growth factor receptor activation to Nox activity remain largely unknown. Nox activation in non-phagocytic cell types varies considerably and continuingly drifts away from the prototypical Nox2 paradigm.49 Various triggers result in Nox-mediated H2O2 generation by either (a) stimulating Nox isoform activity by recruiting or inducing various Nox regulatory subunits, or (b) by triggering Nox isoform expression.

Numerous stimuli increase non-phagocytic Nox expression. Given the large amount of contrasting data, induced expression of particular Noxs appears to be both stimulus and cell type-specific. Nox1 transcription is upregulated by growth factors receptors (platelet-derived growth factor (PDGF)),50 inflammatory mediators (interferon-γ (IFN-γ)), pathogenic molecules (lipopolysaccharide (LPS)), vitamin D3 and hypoxia (HIF-1α). GATA-6 and STAT1 have both been implicated in the transcriptional regulation of Nox1.51 Nox4 expression occurs in a plethora of cell types, including pancreatic, vascular smooth muscle (VSMC) and myeloid cell lines.49 Activators of Nox4 transcription in smooth muscle cells include urokinase, plasminogen activator, angiotensin-II, TGF-β1 and TNF-α.51 Currently, there are little data regarding Nox4-related transcription factors and promoter structures. Duox1 and Duox2 are widely and differentially expressed throughout most human tissues. Induction of Duox1 and Duox2 expression has been elicited in response to Th1 and Th2 dominant cytokines, respectively.52 Interestingly, regulation of Duox expression in animal models by pathways such as cAMP have failed to correlate with human cell lines/tissues.53 Despite studies identifying Duox promoter regions, a void remains pinpointing relevant transcription factors. Expression of Nox3 and Nox5 has been documented in various human tissues; however, characterisation of specific promoter regions and transcription factors remains unchecked.

Upregulation of Nox activity accounts for growth factor-induced ROS production in most cases of Nox pro-survival signalling. Nox1 and Nox3 tend to follow the original Nox2 model such that a stimulus triggers the formation of the active Nox complex, coupled with various combinations of protein subunits. These activating cytoplasmic proteins include p22phox, p47phox and homologue NoxO1, p67phox and homologue NoxA1, and Rac1/2.12 Activation of these subunits appears to rely heavily on phosphorylation by specific kinases. A resultant increase in Nox activity in response to subunit phosphorylation has only been demonstrated in the case of p47phox and Rac. Phosphorylation of p47phox by specific kinases (protein kinase-C (PKC), p38 MAPK, p21-activated kinase), removes its inherent auto-inhibition, allowing p47phox to bind to the cytoplasmic tail of p22phox, activating the Nox complex.54 Rac1/2 is a small RhoGTPase and can be bound to GDP, making it inactive, or to GTP, making it active. Guanine nucleotide exchange factors (GEFs) and guanine-activating proteins (GAPs) promote and inhibit Rac1/2 binding, respectively.14 Nearly 200 different GAPs and GEFs have been identified thus reflecting the complexity of potential Nox activation mechanisms in Rac-dependent Nox1&2 alone. p22phox is known to associate with Nox4; however, whether it is required for ROS generation is uncertain. A novel p22phox-interacting protein, poldip2, has recently been shown to activate Nox4 in VSMC lines.55 By contrast, some groups claim that Nox4 is constitutively active and regulated by expression alone.56 Nox5, Duox1 and Duox2 are activated by increased calcium concentrations, owing to EF-hand Ca2+-binding domains. It has been shown that calcium binding triggers a conformational change at the N-terminal, thus permitting transfer of electrons.57 Jagnandan et al.58 showed that phorbol-12-myristate-13-acetate (PMA)-induced ROS led to the phosphorylation of Nox5, increasing sensitivity to calcium, promoting activation at lower calcium concentrations. Although recent papers describe Nox5 regulation by PKA, PKC, PIP2 and c-Abl, activation of Nox5 by factors other than calcium remains unclear.59 Similar to Nox4 activation, the necessity for Duox–p22phox binding is of uncertain consequence.60

The novel growth factor receptor–Nox relationship continues to show exquisite heterogeneity, currently masking any potential mechanism that may transcend the spectrum of stimuli and Nox isoforms described.

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Future Perspectives

It is clear that Nox-generated H2O2 can have opposing roles in the cell owing to its novel second messenger status. The initiation and propagation of pro-survival signalling in response to ROS relies heavily on subcellular location of Nox enzymes as well as on stimulus and cell type. Several mechanisms exist through which ROS can potentially modulate signalling by oxidation. Importantly, several aspects of ROS signalling remain uncertain. The uncertainty regarding intracellular H2O2 diffusion dynamics demands clarification. The recent aquaporin-3 data coupled with the exquisite specificity of ROS signalling explicitly contradict the widely, and perhaps blindly, accepted ‘freely diffusible’ theory of H2O2. Furthermore, identification of specific ROS protein targets together with compartmentalisation of Nox activity will uncover the unique mechanisms by which Noxs regulate various cellular signalling pathways. Finally, enormous potential exists for therapeutic manipulation of the Nox-driven mechanisms of tumour promotion described earlier. In light of the disappointing performance of antioxidants in the clinical chemotherapeutic arena, targeted Nox isoform inhibition represents a novel anticancer strategy in the future.

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Conflict of interest

The authors declare no conflict of interest.

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Acknowledgements

We are grateful to Professor CJ Chang (Department of Chemistry, University of California, Berkley, CA, USA) for the gift of the Peroxy Orange dye. This work is supported by the Irish Cancer Society, The Children's Leukaemia Research Project Ireland and Science Foundation Ireland.

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivative Works 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/