The Walter and Eliza Hall Institute, Post Office Royal Melbourne Hospital, Victoria 3050, Australia

Correspondence to: D L Vaux, The Walter and Eliza Hall Institute, Post Office Royal Melbourne Hospital, Victoria 3050, Australia E-mail: vaux@wehi.edu.au

Dear Editor,

DIABLO/Smac is a mammalian protein that promotes apoptosis by binding to inhibitor of apoptosis (IAP) proteins.^1,2 Chai et al.³ and Srinivasula et al.⁴ have shown that the N-terminal 4 amino acids or processed DIABLO/Smac are necessary, and the first 7 amino acids are sufficient, for this interaction, just as the N-terminal residues of the insect pro-apoptotic proteins Grim, Reaper and Hid are required for the interaction with insect IAPs.^5,6

Sequence similarity among Grim, Reaper and Hid is restricted to their N-terminal 14 amino acids.⁷ Comparison of their N-terminal sequences with those of processed DIABLO/Smac reveals it also bears similar residues and these residues are conserved in both the bovine and porcine proteins (Figure 1).

The sequences and functions of DIABLO/Smac and the insect apoptosis inducing proteins suggests they are structural as well as functional homologues. It will be interesting to determine whether they interact with analogous sites on mammalian and insect IAPs, and whether, for example, they can compete for binding sites.

1 Du C et al (2000) Cell 102: 33-42, MEDLINE

2 Verhagen A et al (2000) Cell 102: 43-53, MEDLINE

3 Chai J et al (2000) Nature 406: 855-862, Article MEDLINE

4 Srinivasula SM et al (2000) J. Biol. Chem. in press,

5 Wang SL et al (1999) Cell 98: 453-463, MEDLINE

6 Goyal L et al (2000) EMBO J. 19: 589-597, Article MEDLINE

7 Deveraux QL and Reed JC (1999) Genes Dev. 13: 239-252, MEDLINE

Figure 1 Comparison of the N-terminal sequences of pro-apoptotic mammalian and insect IAP binding proteins. Identical residues are highlighted in black, conserved residues in grey. Residues of DIABLO/Smac that are: (a) required for the full-length protein to antagonise XIAP inhibition of caspases; and (b) sufficient as a peptide to antagonise XIAP function are indicated.^3,4