Main

Vascular endothelial growth factor (VEGF) is an essential modulator of angiogenesis during both physiological and pathological hypoxia. It stimulates endothelial cell survival, mitogenesis and cell migration in vitro, and is essential for the generation of new blood vessels during embryonic development and in the adult after injury.1 Furthermore, high VEGF levels correlate with decreased overall and disease-free survival in various solid cancers.2, 3 During tumour growth, VEGF levels increase owing to cellular stress, such as hypoxia, and changes in the tumour microenvironment. VEGF has multiple roles during various steps of tumour progression by stimulating vessel growth to provide the necessary oxygen and nutrients,4 but alterations in VEGF signalling have been suggested to have key roles in driving metastasis5, 6 and modulating cancer stemness through both autocrine and paracrine mechanisms.7

p53 is a central component of most cellular stress responses, including hypoxic stress.8 Upon activation, p53 can positively or negatively regulate the expression of numerous target genes involved in various essential cellular processes, including cell proliferation, survival and angiogenesis.9

Despite putative cross-talk mechanisms between p53 and VEGF, how these two key cancer-signalling pathways functionally interact remains unclear. While Agani and co-workers reported10 that VEGF mRNA levels remain unchanged following activation of endogenous or exogenous p53 in Hep3B human hepatoblastoma or in RKO human colorectal carcinoma cell lines, others have shown that wild-type (WT) p53 can repress VEGF transcription in vitro.11 p53 has been shown to repress VEGF transcription by direct binding and sequestration-positive transcriptional regulator SP1 and/or binding to the E2F transcription factor forming a transcriptional repressor complex for VEGF expression.12, 13, 14

Mdm2 and HIF-1α, key modulators of the p53 and VEGF pathways, respectively, further complicate the situation. Under hypoxic conditions, HIF-1α protein is stabilized allowing direct binding to and activation of the VEGF promoter.15 Although evidence exists that during hypoxia HIF-1α can directly interact with and stabilize WT p53 protein,16 others have shown that HIF-1α does not directly interact with p53 and the HIF-1α–p53 interaction occurs only in the presence of Mdm2. Mdm2-dependent p53–HIF-1α interaction decreases Mdm2-mediated degradation and nuclear export of p53.17 At the same time, p53 promotes Mdm2-mediated ubiquitylation and proteasomal degradation of HIF-1α.18 This is in contradiction with the role of Mdm2 as an HIF-1α activator during hypoxia in tumour cells.19

To understand this cross-talk between p53 and VEGF regulation under hypoxic conditions further, we have performed in vitro experiments using non-transformed primary mouse embryonic fibroblasts (MEFs) and in vivo xenotransplantation assays. We observed that p53 has contrasting roles in the regulation of VEGF expression during hypoxia. Unexpectedly, we find that p53 cooperates with HIF-1α to positively regulate VEGF expression during initial acute hypoxic conditions. However, after prolonged exposure to hypoxia or when cells are exposed to genotoxic stress, p53 represses VEGF expression in a p21/retinoblastoma (Rb)-dependent manner. Accordingly, activation of p53 in cells deficient for p21/Rb pathway leads to a sustained increase in VEGF expression and promotes angiogenesis in xenograft models of retinoblastoma. As the p21/Rb tumour suppressor pathway is defective in a high proportion of solid tumours, including retinoblastoma, these findings indicate that chemical reactivation of p53 in such tumours should be undertaken with caution owing to the positive transcriptional effects of p53 on VEGF expression under hypoxia conditions in these tumours.

Results

p53 induces VEGF transcription during the initial stages of hypoxia

Interpretation of the putative cross-talk between p53 and VEGF signalling has so far been complicated owing to the use of cancer cell lines that have accumulated many uncharacterized oncogenic lesions due to genomic instability. Here we have revisited the role of p53 in VEGF transcriptional regulation in non-transformed cells. To this end, we exposed various genetically engineered primary MEFs to hypoxia (0.5% O2). We monitored VEGF expression by quantitative reverse transcription-polymerase chain reaction (qRT-PCR) at an early (4 h) and a late (24 h) time point during hypoxia exposure. VEGF expression levels showed a threefold increase at 4 h and a significant decrease at 24 h (Figure 1a and Supplementary Figure 1A). The hypoxia-regulated PFK promoter, which contain HIF-1α-binding sites, served as the control for hypoxia induction (data not shown).20

Figure 1
figure 1

p53 induces VEGF transcription during the initial stages of hypoxia. (a and b) Relative VEGF mRNA levels measured by qRT-PCR (n=3). (a) WT MEF cells displaying a 3.2-fold induction of VEGF mRNA after 4 h hypoxia. p53−/− MEFs display significantly less induction of VEGF mRNA upon hypoxia. (b) VEGF mRNA levels in WT MEFs do not change by stabilizing p53 with doxorubicin in normoxic (20% O2) conditions. However, the hypoxic induction is decreased upon activation of p53 via doxorubicin. (c) Western blot showing increased expression of p53 only upon doxorubicin treatment and not with hypoxia alone. (d) Western blot displaying p53 levels after Cre excision in Mdm2−/−, p53 LSL/− MEFs. (e) Relative VEGF mRNA levels were measured by qRT-PCR. There is a significant increase in expression after Cre excision and re-induction of p53 (2KO+C) (n=4 for both 2KO and 2KO+C cells) that is further increased in the absence of Mdm2. (f) Relative picogram VEGF protein measured by enzyme-linked immunosorbent assay (ELISA) and normalized against a microgram of cell lysate. Cells display higher levels of protein after re-activation of p53 in the absence of Mdm2. Bars represent mean±S.E.M.; NS, nonsignficant; *P⩽0.05, **P⩽0.01 and ***P⩽0.005

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Unexpectedly, at the early time point, hypoxia-dependent VEGF induction was significantly attenuated in p53-null MEFs, indicating that p53 contributes to this upregulation (Figure 1a). Hypoxia and DNA damage induce p53 via distinct mechanisms and activate different p53 transcriptional programmes and biological responses. To test whether DNA damage-induced p53 stabilization promotes induction of VEGF, we induced p53 using doxorubicin (0.4 μg/ml)21 in normoxic and hypoxic conditions. We found that doxorubicin-induced p53 stabilization is not sufficient to promote VEGF induction in normoxic conditions; it even decreased the hypoxic-dependent induction of VEGF mRNA levels (Figure 1b). In contrast to DNA damage, hypoxia alone does not stabilize p53 as reported previously,22 and only showed increased and stabilized expression when the cells were treated with doxorubicin as well (Figure 1c). These data indicate that p53 has a positive role in the initial induction of VEGF in response to hypoxia, but not in response to DNA damage alone. Moreover, DNA damage interferes with the ability of p53 to induce VEGF expression in hypoxic conditions in WT MEFs. Importantly, p53 also contributes to the downregulation of VEGF expression at the late time point. This is consistent with previous reports that p53 can directly repress the VEGF promoter.11

To further validate the positive effects of p53 on VEGF expression, we used MEFs harbouring a lox-stop-lox (LSL) transcription cassette inserted into the first intron of p53 and also lacks the p53-negative regulator Mdm2 (Mdm2−/−, p53 LSL/− hereafter referred to as 2KO (knockout) cells). Consistent with a previous study,23 Cre-mediated excision of the LSL cassette leads to an accumulation of p53 in the absence of exogenous DNA damage (Figure 1d). Following exposure to hypoxia, reactivation of p53 (Mdm2−/−, p53+/−, hereafter referred to as 2KO+C) caused a robust increase in VEGF levels after 4 h (Figure 1e). This genetic rescue experiment confirms that p53 promotes the initial induction of VEGF expression during hypoxia (Figure 1b). VEGF mRNA levels were further increased at 24 h, indicating a negative role of Mdm2 in the modulation of VEGF expression under conditions of extended hypoxia (Figure 1e). Consistent with the mRNA expression data, 2KO+C cells show higher protein levels compared with 2KO cells (Figure 1f). Therefore, we conclude that p53 is able to induce VEGF during the initial phases of hypoxia and our data are consistent with previous reports of the negative role of Mdm2 on HIF-1α function.18

p53 binds to VEGF promoter synergistically with HIF-1α to induce VEGF expression during early hypoxia

To determine whether p53 directly binds to the VEGF promoter and leads to increased VEGF mRNA expression during early hypoxia, we performed an in silico promoter analysis of the VEGF promoter using the ConTra algorithm.24 We identified three highly conserved putative p53-binding sites. Region 1 (R1) contains a conserved double p53-binding site, one part being directly adjacent to the well-characterized, highly conserved and functional HIF-1α-binding site,25 while the other p53-binding site lies 64 base pair (bp) upstream. Region 2 (R2) is located in the first intron where the p53 and HIF-1α sites overlap and region 3 (R3) is another double p53 site with a 9 bp spacer and is also located in the first intron (Figure 2a and Supplementary Figure 2).

Figure 2
figure 2

p53 directly binds to VEGF promoter during early hypoxia and is dependent on the presence of HIF-1α. (a) Schematic view of the conserved p53 and HIF-1α regions analysed by chromatin IP. HIF-1α (red), p53 (blue) in R1, R2 and R3. (b) Chromatin IP on VEGF promoter using p53 antibody (Ab) in MEFs with different genetic backgrounds after 4 h of hypoxia. p53 binds to VEGF promoter in WT and 2KO+C MEF cells but fails to bind in HIF-1α−/−, and p53−/− MEF cells. (c) VEGF promoter luciferase assay (n=3). Luciferase expression only in WT cells that contain complete promoter sequence. Bars represent mean±S.E.M.; NS, nonsignificant; **P⩽0.01

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To investigate the functionality of these putative p53-binding sites, we performed chromatin immunoprecipitation (ChIP) experiments. Primers were designed to flank the putative p53-binding sites R1, R2 and R3. In WT cells, p53 binds to R1, but not R2 or R3, after 4 h of exposure to hypoxia. p53 recruitment to R1 was observed in the 2KO+C cells but not in HIF-1α null cells, indicating that while the presence of HIF-1α is required for p53 binding, Mdm2 protein is not necessary for the binding of p53 to the VEGF promoter at R1 region19 (Figure 2b). To further confirm that this R1 site is required for hypoxia-dependent VEGF induction, we transfected VEGF promoter luciferase constructs, which contains 1.6-kb fragment of VEGF genomic DNA, encompassing 1.2 kb of the 5′-flanking sequence of the transcription start site and 0.4 kb of corresponding 5-UTR26 into WT, p53-null and HIF-1α-null MEFs, and exposed these cells to hypoxia for 4 h. While hypoxia induced luciferase activity in WT cells, it did not in p53- or HIF-null MEF cells (Figure 2c). Mutation of either the p53 or HIF-1α putative binding sites in R1 (referred to Δp53 and ΔHIF-1α, respectively) in the VEGF promoter abrogated the hypoxia-induced luciferase activity (Figure 2c). These results indicate that hypoxia-induced VEGF expression requires the co-recruitment of both HIF-1α and p53 to the R1 site during the initial stages of hypoxia.

p21 is involved in repressing VEGF levels during continued hypoxia

In addition to its positive role in hypoxia-induced VEGF expression at early time points, p53 also functions as a negative regulator of VEGF at later time points. p53-mediated transcriptional repression can either be direct or indirect via induction of microRNAs26 or induction of the CDK inhibitor p21 whose activity affects E2F levels,27 or by directly binding with E2F, which in turn has been implicated in the transcriptional repression of VEGF.13 To investigate the involvement of p21 in VEGF transcriptional repression, p21-null MEFs were subjected to the same hypoxic conditions as described above. As expected, a significant increase in VEGF levels was observed at 4 h and, importantly and in contrast to WT and p53-null MEFs, this induction was sustained at 24 h (Figure 3a). Moreover, DNA damage-induced p53 failed to repress VEGF expression in the absence of p21 (Figure 3b). p21-null cells also showed higher VEGF protein levels under hypoxic conditions with and without doxorubicin (Figures 3c and d). In addition, the kinetics and extent of hypoxia-induced VEGF expression in MEFs with a dysfunctional Rb pathway (Rb/p107-null, two key negative regulators of E2Fs activity) was comparable to those seen in p21-null MEFs (Figure 3e).

Figure 3
figure 3

p21 is required for the repression of VEGF expression during extended hypoxia. (a and b) Relative VEGF mRNA levels measured by qRT-PCR (n=3). (a) VEGF mRNA is induced in p21−/− MEFs and this induction is stable after 24 h hypoxia. (b) VEGF expression is induced even upon activation of p53. (c and d) Relative picogram VEGF protein measured by enzyme-linked immunosorbent assay (ELISA) and normalized against a microgram of cell lysate (n=3). P21−/− MEFs display the highest levels of VEGF protein after hypoxia and stabilization of p53 by doxorubicin. (e) Relative VEGF mRNA levels measured by qRT-PCR. VEGF expression is not repressed in pRb/107−/− MEFs after 24 h of hypoxia, displaying a similar trend as p21−/− MEFs. (f) Western blot on WT cells displaying levels of p21 and E2F during hypoxia and hypoxia combined with doxorubicin. Bars represent mean±S.E.M.; NS, nonsignificant; *P⩽0.05, **P⩽0.01, ***P⩽0.005, ****P≤0.001

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Despite the fact that p21-deficient cells showed a lack of VEGF repression at the later time points of hypoxia, the overall p21 mRNA and protein levels were not changed under hypoxic conditions alone but only upon doxorubicin- or Nutlin-induced stabilization of p53 (Figure 3f and Supplementary Figures 1A and 3A, B). Moreover, only in 2KO+C cells, where the overall steady-state levels of p53 are increased, does prolonged hypoxia for 24 h result in increased p21 mRNA levels (Supplementary Figure 3C). Use of the Mdm2 inhibitor Nutlin-3 did not affect the kinetics of VEGF mRNA induction during the initial 4 h of hypoxia observed in WT, p53-null or p21-null MEFs (Supplementary Figure 1A). However, 24 h of hypoxia combined with Nutlin-3 treatment in WT cells led to a marginal decrease in VEGF expression, whereas p21-null cells remained unaffected. VEGF protein levels continued to accumulate during the 24 h period of hypoxia and were highest in all conditions in p21-null cells (Supplementary Figure 1B).

Surprisingly, the levels of E2F protein, known to have a role together with p53, in the transcriptional repression of VEGF,13 showed bi-phasic protein expression levels, whereby during initial hypoxia E2F levels were decreased but were increased during extended hypoxia (Figure 3f). This was irrespective of p53 stabilization by doxorubicin (Figure 3f). However, the increased E2F protein expression at 24 h of hypoxia was less pronounced upon continued Nutlin treatment (Supplementary Figure 3B). Here hypoxia alone did not change the phosphorylation status of Rb, whereas hypophosphorylated (active) Rb was greatly increased upon doxorubicin- or Nutlin-3-induced stabilization of p53 and activation of p21, leading ultimately to decreased Ser10 histone H3 phosphorylation (Supplementary Figure 3B) that has been linked to cell-cycle arrest.28, 29

To confirm p21 involvement in VEGF mRNA repression during extended hypoxia, rescue experiments in p21-null MEFs were performed by using viral transduction of an HA-tagged human p21 construct (Supplementary Figure 3D). We could show that the repressive activity on VEGF mRNA expression in these cells after 24 h of hypoxia was restored (Supplementary Figure 3E).

p21-null MEFs enhance vascularization in xenotransplanted tumours

There is accumulating evidence that tumour-associated fibroblasts have important roles in tumour growth by releasing key angiogenic cytokines/growth factors.30, 31 To further assess the suppressive role of p21 on VEGF regulation and its potential implications on tumour angiogenesis, we investigated whether p21-deficient MEFs could enhance tumour angiogenesis compared with WT MEFs, using xenotransplantation experiments. Here HCT116 tumour cells32 WT for p53 were combined with p21-null or WT MEFs in a 3 : 4 ratio and injected subcutaneously in athymic nude (NMRI nu/nu) mice. Tumours injected with p21-null MEFs displayed more vessels than their WT counterparts and displayed fewer hypoxic regions at the tumour periphery (Figures 4a and b and Supplementary Figures 4A and B). In HCT116 tumours derived with WT MEFs, the vasculature was confined to the periphery with tumours displaying large central necrotic areas (Figure 4a and Supplementary Figure 4A). HCT116 tumours derived with p21-null MEFs had increased vessels surrounding the tissue periphery and at the centre of the tumour, resulting in fewer necrotic regions (Figure 4b). Quantification of vessel density showed that the HCT116 tumours derived with p21-deficient MEFs had a 50% increase in vessel density compared with HCT116-derived tumours that were co-injected with WT MEFs (Figure 4c; P=0.03). This result was correlated with an approximate twofold increase in VEGF levels (Figure 4d; P=0.07). This observation indicates that p21-null MEFs have a positive effect on tumour angiogenesis through enhanced expression of VEGF.

Figure 4
figure 4

p21-null MEFs induce vascularization in xenotransplanted tumours. (a and b) Brightfield montaged × 40 and × 100 images of tumour sections stained with platelet endothelial cell adhesion molecule (PECAM). (a) HCT116 injected with WT MEFs. Most vessels surround the tumour tissue, with fewer vessels in the centre. Necrotic region is represented by asterisk. (b) HCT116 cells with p21−/− MEFs. High density of vessels at the surrounding and the centre of the tissue. (c) Quantification of vessels using the Volocity software program. The tumours injected with p21−/− MEFs (n=18) on average contain more vessels than their WT counterparts (n=17); n represents the total number of slides for five and three tumours for p21−/− and WT, respectively. (d) Relative VEGF mRNA levels measured by qRT-PCR (n=3). Tumour samples with p21−/− MEFs display more VEGF expression compared with their WT counterparts. Bars represent mean±S.E.M.; *P⩽0.05

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WT p53 promotes angiogenesis in retinoblastoma xenografts

The observation that p53, in addition to its indirect repressive role in dampening VEGF expression via the p21/Rb pathway, actually promotes the initial induction of VEGF has significant implications for tumourigenesis. In tumours with a dysfunctional p21/Rb pathway, p53 activation may promote VEGF expression via HIF-1α, eventually leading to enhanced angiogenesis and increased tumour growth.

Retinoblastoma cells provide an ideal model to test this possibility as the Rb and p53 pathways are, respectively, dysfunctional and intact in these cells.33 To compare angiogenesis in the presence and absence of p53, we knocked down (KD) p53 in the human Y7934 (Y79 short hairpin (sh)p53) and WERI35 (WERI shp53) retinoblastoma cell lines that harbour WT p53 (Figure 5a and Supplementary Figure 6A). These cells, and control KD cells (Y79 shMX, WERI shMX), were injected intravitreally into NMRI nu/nu mice and subsequently monitored for the presence of a white reflex behind the pupil, an indicator of tumour growth. Tumours were collected 14 days after engraftment and stained for the endothelial marker CD31 as well as hypoxia. The Y79 and WERI shMX control eye tumours displayed more vessels than tumours arising from the Y79 and WERI p53 KD cells (Figures 5b and c and Supplementary Figures 5 and 6). These tumours were characterized by fewer necrotic regions and overall less hypoxia (Figures 5b and c and Supplementary Figures 5 and 6). Quantification of vessel densities for the Y79 tumours showed a highly significant 50% reduction (P=0.001) in the p53 KD tumours (Figure 5d), which correlated with an approximate 1.5-fold decrease in VEGF levels (Figure 5e; P=0.09). These results provide in vivo evidence that p53 has a positive effect on tumour angiogenesis in Rb1-deficient tumours.

Figure 5
figure 5

WT p53 promotes angiogenesis in retinoblastoma xenografts in the absence of an intact Rb pathway. (a) Western blot on Y79 cells showing knock down of p53 with short hairpin. (b and c) Brightfield montaged × 40 images and computer-enlarged boxed regions of eye sections stained with PECAM. (b) Y79 shp53. (c) Y79 shMX. (d) Quantification of vessels using the Volocity software program. Y79 shp53 (n=17) on average contain less vessels than Y79 shMX (n=15); n represents the total number of slides for six and seven tumours for Y79 shMX and Y79 shp53, respectively. (e) Relative picogram VEGF protein measured by enzyme-linked immunosorbent assay (ELISA) and normalized against a microgram of cell lysate (n=3). Tumours with p53 short hairpin on average display less VEGF protein than their control counterparts. Bars represent mean±S.E.M.; ****P⩽0.001

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Discussion

Both p53 and VEGF have been shown to have essential roles in cancer formation. Previous conflicting results concerning the regulation of VEGF by p53 can be attributed to the fact that previous experiments were performed on various tumour cell lines with different mutational profiles and response to hypoxia with respect to VEGF regulation. In addition, some of these in vitro studies were performed using transient expression assays that are sensitive to variations in experimental set-up.10, 14 For this study, we used non-transformed primary MEFs from different genetic knockout backgrounds, which are more representative of physiological conditions. Using this approach, we have shown that p53 positively regulates VEGF expression during early stages of hypoxia and that Rb/p21 acts indirectly to repress VEGF expression in primary non-transformed cells independently of effects on Rb phosphorylation.

Intriguingly, we found that p53-null cells showed a blunted VEGF mRNA response after 4 h of hypoxia. This does not contradict the fact that p53-null tumours are typically highly angiogenic. Overall, we observed increased levels of VEGF protein at 24 h of hypoxia in p53-null compared with WT cells (Supplementary Figure 1). It is important to note that although p53-null cells do not show initial induction, they also do not possess repressive activity on VEGF promoter activity, and therefore as long as the hypoxia state exists, there will be a constant steady state of VEGF mRNA production and accumulation that will be translated to increased VEGF protein. Here post-transcriptional/translational modifications that increase the stability of VEGF mRNA and protein during hypoxia should be taken into account.36

In this study, we have demonstrated that in the absence of Rb/p107, VEGF levels are no longer repressed during prolonged hypoxia in primary MEFs. Second, we have demonstrated that p21-null MEFs also show a lack of repression of VEGF mRNA and protein under conditions of prolonged hypoxia, irrespective of the stabilization of p53. Under hypoxic conditions alone however, p53 and p21 protein levels are not changing and only upon DNA damage or Mdm2 inhibition are the protein levels of p53 and p21 increased. The lack of hypoxic induction of p53/p21 is consistent with previous reports;8, 22 however, this lack of protein induction does not rule out changes in p53 transcriptional or p21 kinase activity towards their differential targets. Last, we have shown that E2F protein expression is lower during the initial phases of hypoxia than during prolonged hypoxia. This observation is consistent with the early induction and late repression of VEGF during hypoxia and with the findings by Qin and co-workers,13 who showed that E2F/p53 complex attenuates VEGF expression and that E2F-null mice display more angiogenesis compared with their WT counterparts.13

During normal cell-cycle regulation, cytotoxic stress induces p53 expression and stabilization, which in turn leads to increased p21 levels. p21 then represses the phosphorylation of Rb and decreases the amount of free E2F that can activate cell-cycle genes. However, under prolonged hypoxia p21 levels are not changing and the phosphorylation of Rb remains unchanged, yet there is apparently more E2F under extended hypoxic conditions to potentially act synergistically with p53 to repress VEGF.13 These data lead us to conclude therefore that the p21/E2F/Rb pathway acts as an indirect repressor of VEGF expression under conditions of continued hypoxia that are mechanistically distinct from the classical roles of the p21/E2F/Rb pathway in cell-cycle regulation.

Despite the lack of changes in its steady-state levels during extended hypoxia, our genetic deletion data clearly point to a role of p21 as an inhibitor of VEGF and the angiogenic programme. This is consistent with p21-null mice developing vascular tumours37 that may be related to increased VEGF levels. In keeping with this hypothesis, we have also demonstrated that when co-injected with human HCT116 cell line, p21-null MEFs can drive an enhanced tumour angiogenic response that correlated with enhanced VEGF expression levels.

Our finding using retinoblastoma models where the continued presence of p53 in the absence of a functional Rb pathway results in more vascularized tumours is novel and biologically relevant. It is known that retinoblastomas are highly angiogenic tumours and that vessel density correlates with both its invasiveness and metastatic potential.38, 39 In case studies where patients with aggressive retinoblastoma failed to benefit from systemic chemotherapeutic treatment, biopsy samples showed upregulation of the VEGF pathway stressing the relevance of this pathway with poor prognosis.38 Our findings provide further insight into why these tumours are so highly angiogenic and caution against reactivation of p53 as the sole treatment in these Rb-deficient tumours. For instance, tumours treated with Nutlin-3 (Mdm2 antagonist), which would stabilize WT TP53, fail to regress and a reduction of tumour burden is only observed in combination with another drug (topotecan, a topoisomerase I inhibitor).33

In conclusion, the stress response protein p53 interacts synergistically with the physiological regulator HIF-1α40 to promote VEGF expression during acute hypoxia to adjust perturbations in oxygen and nutrient homoeostasis and indirectly regulates cellular metabolism.41 However, during prolonged hypoxic exposure, which is also observed in tumours, p53 acts as a repressor to prevent VEGF induction and inhibits angiogenesis in a p21/Rb-dependent manner. These findings have significant therapeutic implications for WT p53 tumours carrying a disrupted Rb/p21 pathway. Here, enhanced VEGF levels could fuel vessel abnormalization,4 increase tumour lymphatic metastatic potential42 and/or potentially increase cancer stem cell numbers in the tumour,7 thereby exacerbating tumour refractiveness to chemo- and radiation therapy. Thus, p53 stabilization therapies aimed at killing tumour cells should only be considered in the context of evidence of an intact p21/Rb pathway or should be combined with strategies that may normalize tumour vasculature that can act in synergy with stabilized p53 in enhancing tumour cell apoptosis.

Materials and Methods

Cell culture and conditions

MEFs were isolated from mouse embryos according to the protocol described in Migliorini et al.43 Human colorectal carcinoma cell line, HCT116, was kindly provided by Prof. B Vogelstein (Johns Hopkins University, Baltimore, MD, USA). MEFs and HCT116 cells were grown in DMEM supplemented with 10% FBS, 2 mM L-glutamine, 0.1 mM non-essential amino acids, 50 μM β-mercaptoethanol, and 50 U/ml penicillin and streptomycin. Human retinoblastoma cell lines Y79 and WERI and clones with shp53 were grown in RPMI 1640 medium supplemented with 10% FBS, and 50 U/ml penicillin and streptomycin.

Hypoxia setting

MEFs at passage 2 (P2) were grown in normoxic condition (20% O2, 5% CO2). At D0 the cells are splitted (P3) and counted for a confluency of approximately 70% for the non-treated and hypoxia 4 h samples and 50% confluency for the hypoxia 24 h samples. At D1 the medium is refreshed and cells are placed in hypoxic chamber (0.5% O2, 5% CO2). After 4 h, the non-treated and hypoxia 4 h cells are harvested for RNA and protein, and the supernatant is collected for ELISA. The same procedure is also applied for the hypoxia 24 h samples.

Hypoxyprobe

To detect tumour tissue hypoxia mice are injected intraperitoneally with Hypoxyprobe-1 Kit (HPI Inc., Burlington, MA, USA) at a dosage of 60 mg/kg body weight at 90 min before being sacrificed. The tumours are then dissected and processed as explained in the histology section. For hypoxia detection on the paraffin-fixed tissues, Hypoxyprobe-1 Kit (HPI Inc.; no. HP1-100Kit) was used according to the manufacturer’s protocol.

Histology

Tumours were fixed overnight in 4% paraformaldehyde at 4 °C, processed for paraffin embedding and sectioned at 5 μm. Sections were stained with haematoxylin and eosin. Immunohistochemistry was performed according to the protocol from Renaissance TSA Biotin System (NEL 700; Perkin-Elmer, Waltham, MA, USA), using rat-anti-mouse CD31 antibody (BD Biosciences, Erembodegem, Belgium; 1 : 50), followed by incubation with biotinylated anti-rat antibody (BD Biosciences).

Chromatin immunoprecipitation

ChIP assays were performed using protocol adapted from Ohkubo et al.44 Briefly, subconfluent 15 cm dishes were crosslinked in 1% formaldehyde for 10 min at room temperature. The reaction was stopped by the addition of glycine in PBS to a final concentration of 125 mM. Cells were transferred on ice, rinsed three times with ice-cold 1 × PBS and scraped in PBS with 1 mM EDTA and protease inhibitors. Lysates were centrifuged for 10 min at 4 °C at 4000 r.p.m.; further cell pellets were resuspended in 1 ml ChIP lysis buffer (0.1 M PIPES, pH=8, 1 M KCl, 10% NP40) with protease inhibitor and 0.5 mM PMSF, and centrifuged for additional 5 min at 4 °C at 5000 r.p.m. Finally, pellets were resuspended in 1 ml of sonication buffer (1 M Tris-HCl, pH=8, 0.5 M EDTA, 20% SDS, 10% deoxycholic acid) with protease inhibitor, and subsequently sonicated at high power in a Bioruptor sonicator, Liėge, Belgium (cycle of 3 × 10 min sonication, 3 × 10 min pause; interval 30 s). Samples were centrifuged at 4 °C, and the supernatants were transferred to a new tube. The DNA concentration was determined and aliquots of DNA were taken to represent input material. For 100 μg of DNA chromatin solution, 3 μg of anti-p53 antibody (mouse, clone 1C12; Cell Signalling Technology, Leiden, The Netherlands; no. 2524 lot 4) was used. IPs were performed for 2 h at 4 °C using 50% slurry of protein G beads with 0.1 μg/μl BSA, and 0.1 μg/μl salmon sperm. Beads were subsequently washed three times in wash buffer (10 mM HEPES, pH=7.6, 0.5% Nonidet P-40, 1 mM EDTA, 200 mM NaCl, 10% glycerol, supplemented with protease and phosphatase inhibitors). Elution of the crosslinked material was performed by incubating the beads, while rotating, for 20 min at room temperature in elution buffer (1% SDS, 0.1 M NaHCO3). After centrifugation, the supernatant was transferred into a new tube. A measure of 16 μl of 5 M NaCl was added to the samples and to the input chromatin (50 μl+350 μl elution buffer). All the samples were de-crosslinked overnight at 65 °C. Chromatin DNA was purified using QIAquick PCR Purification Kit (Qiagen, Venlo, Netherlands; no. 28104), according to the manufacturer’s protocol. Finally, both the input and co-precipitated DNA were analysed using real-time qPCR. The following genes were used as reference: β-actin, hydroxymethylbilane synthase (HMBS), glyceraldehyde-3-phosphate dehydrogenase (Gapdh), VEGF R1, VEGF R2 and VEGF R3. Primers used to amplify the specific genomic regions are shown in Supplementary Table 1.

Lentivirus production, cell infection and selection

HEK293T cells were transfected by calcium phosphate for 6 h with a mix of vectors pSPAX2, pMD2G (Trono Lab, Lausanne, Switzerland), pDG2iV5puro encoding an N terminus 3 × HA-tagged human p21. Multicistronic pDG2iV5puro is a modified version of the pLenti6 V5/DEST vector (Invitrogen, Life Technologies Europe B.V., Gent, Belgium). It contains an expression cassette under the control of a Tet-responsive element allowing the doxycycline-inducible expression of the gene of interest and an SV40 promoter controlling rtTA3 transactivator expression, followed by an IRES and a puromycin-resistance marker. Transfected HEK293T supernatant was collected after 2 days, clarified, filtered and concentrated on Amicon filter (Millipore, Overijse, Belgium). MEF p21-null cells were infected for 24 h by the addition in culture medium of a volume of lentiviruses corresponding to 100 μg of p24 capsid protein per 50.103 cells (titrated by ELISA according to the manufacturer’s protocol; INNOTEST HIV Antigen mAb; Innogenetics, Gent, Belgium). Infected cells were selected by antibiotic resistance with a final concentration of 1.5 μg/ml of puromycin (InvivoGen, Toulouse, France), 24 h before hypoxia experiments cells are treated with 1 μg/ml doxycycline to activate ectopic p21 expression.

Western blot

MEFs were dissolved in Giordano buffer (50 mM Tris-HCl, pH 7.4, 250 mM NaCl, 0.1% Triton X-100, 5 mM EDTA) or 1 × Laemmli buffer (10% glycerol, 100 mM DDT, 62.5 mM Tris) containing phosphatase and protease inhibitors (Sigma-Aldrich BVBA, Diegem, Belgium). The protein concentration was determined by Bio-Rad DC Protein Assay (Bio-Rad Laboratories N.V., Nazareth Eke, Belgium; no. 500-0112) and 30 μg of each sample was fractionated by SDS-PAGE and transferred to a polyvinylidene difluoride (PVDF) membrane (Amersham Biosciences, GE Healthcare Europe Gmbh, Diegem, Belgium). The primary antibodies used were as follows: anti-p53 (mouse, clone 1C12, 1 : 1000; Cell Signalling Technology ; no. 2524 lot 4), anti-p21 (rabbit, clone C-19, 1 : 200; Santa Cruz, Biotechnology, Inc., Heidelberg, Germany; no. sc-397), anti-β-tubulin (rabbit, 1 : 1000; Abcam, Cambridge, UK; no. ab6064), anti-E2F1 (rabbit, clone c-20, 1 : 250; Santa Cruz Biotechnology; no. sc-193), anti-phosphohistone H3 (Ser10) (rabbit, 1 : 200; Cell Signalling Technology; no. 9701), anti-α-tubulin (mouse, clone DM1A, 1 : 1000; Santa Cruz Biotechnology; no. sc-32293), and anti-Rb (mouse, 1 : 500; BD Pharmingen, Erembodegem, Belgium; no. 554136), anti-phospho-Rb (pT826) (rabbit, 1 : 500; Biosource Invitrogen, Gent, Belgium; no. 44-576G) and Vinculin (human, clone hV1N-1, Diegem Belgium, 1 : 1000; Sigma-Aldrich BVBA; no. V9131). WERI cells were lysed with Triton 1% lysis buffer (50 mM HEPES, pH=7.5, 150 mM NaCl, 1 mM EDTA, 2.5 mM EGTA) containing phosphatase and protease inhibitors (Sigma-Aldrich BVBA). Primary antibodies used were as follows: anti-p53 (mouse, clone DO-1, 1 : 1000; Santa Cruz Biotechnology; no. sc-126) and anti-GAPDH (mouse, clone 6C5, 1 : 1000; Abcam; no. ab8245). The secondary antibodies used were either horseradish peroxidase (Amersham Biosciences) or IRDye 680 anti-mouse IgG (goat, 1 : 10 000; Li-Cor, Bad Homburg, Germany; no. 926-32220) and IRDye 800CW anti-rabbit IgG (goat, 1 : 10 000; Li-Cor; no. 926-32211). For the detection, Li-Cor Odyssey infrared imaging system (Li-Cor) was used.

ELISA

Secreted VEGF was measures from the supernatant of cell cultures using Mouse VEGF Quantikine ELISA Kit (R&D Systems Europe Ltd., Oxon, UK; no. MMV00) according to the manufacturer’s instructions. Values were normalized against total protein concentration of cell lysates.

Microscopy and vessel quantification of tumours

For whole images of tumour and eye sections, AxioVision 4.8.2 (Carl Zeiss N.V., Zaventem, Belgium) was used. Tumours were isolated when they reached the size of 2 cm3. Sequential sectioning of paraffin-embedded tumours were performed for three WT and five p21-null samples or six Y79 shMX control eye tumours and seven of Y79 p53 mutant tumours. Vessel growth was analysed using CD31 immunoreactivity and quantificated using the Volocity software program (Perkin-Elmer).

Statistical analysis

All statistical claims are based on standard paired Student’s t-test.

Xenotransplantation assay

All animal experiments were approved by the Ethical Committee for care and use of laboratory animals of the University of Ghent. HCT116 cells were injected with either p21-null or WT cells with a ratio of 3 : 4 subcutaneously in the flank of athymic nude (NMRI nu/nu) mice. For retinoblastoma model, 6000 cells of Y79/or WERI shMX as control (short hairpin against mouse MdmX in a human tumour cell line) and Y79/or WERI shp53 were injected intravitreally in the eyes of athymic nude (NMRI nu/nu) mice. For retinoblastoma model of WERI cells, the same procedure as Y79 cells was performed.

In vitro Cre excision

An HTNC-expressing Escherichia coli strain, created by Peitz et al.,45 was used for the expression of the modified Cre recombinase, which was purified using an Ni-NTA resin column and concentrated by dialysis. For Cre-mediated deletion, MEF culture was treated with HTNC-containing MEF medium without FBS for 8 h. Subsequently, cells were washed with 1 × PBS and refreshed with the MEF medium.

Quantitative reverse transcription-polymerase chain reactions

Total RNA was isolated using RNeasy Plus Mini Kit (Qiagen). cDNA was synthesized using the First Strand cDNA Synthesis Kit (Roche Diagnostics Belgium, Vilvoorde, Belgium) with oligo(dT) primer by starting from equal amounts of RNA as measured by a NanoDrop spectrophotometer (Thermo Scientific, Doornveld, Belgium). qRT-PCRs were performed using the LightCycler 480 SYBR Green I Master (Roche) and monitored on a LightCycler 480 system (Roche). Gene expression was standardized against housekeeping genes β-actin, HMBS and Gapdh. All qRT-PCR primers that were used are listed in Supplementary Table 2.

Luciferase activity assay

Dr. Patricia D’Amore (Laboratory Schepens Eye Research Institute, Boston, MA, USA) kindly provided VEGF promoter luciferase construct.26 MEFs were transfected with VEGF promoter luciferase construct and pUT651 (Eurogentec S.A., Seraing, Belgium) as control with 4 : 3 ratio, respectively. Non-treated samples were kept at 20% O2 and others were exposed to hypoxia for 4 h. Luciferase activity was measured using the Galactostar Kit (Tropix, Life Technologies Europe B.V.) according to the manufacturer’s protocol.

Bioinformatic analysis

Putative transcription factor-binding sites in the VEGFA promoter and intron 1 region were identified using the web-based analysis tool ConTra (Broos et al.,46). In a Multiz alignment of the promoter (2000 upstream) and first intronic region of VEGFA from different species, highly conserved binding sites were detected for p53, HIF1-α. Sites were identified based on the position weight matrices from the JASPAR (Copenhagen University, Ole Maaloes Vej 5, Denmark) and TransFac (Wolfenbuettel, Germany); databases. The JASPAR matrices included MA0259.1 (HIF-1). From TransFac three matrices were used as follows: V$P53_02 and V$P53_DECAMER_Q2 (p53), and $HIF1_Q3 (HIF-1α). Three regions containing highly conserved p53-binding motifs, of which two also included conserved HIF-1 sites, were experimentally validated: R1 in the promoter region on human chr6: 43 736 903–43 736 999 and on mouse chr17: 46 170 223–46 170 318 (Figure 2 and Supplementary Figure 2); R2 in intron 1 on human chr6: 43 739 265–43 739 313 and on mouse chr17: 46 167 974–46 168 022 (Figure 2 and Supplementary Figure 2); and R3 in intron 1 on human chr6: 43 740 348–43 740 405 and mouse chr17: 46 166 840–46 166 895 (Figure 2 and Supplementary Figure 2). Figures were generated with the program Jalview using the multifasta alignment and feature colour files produced by the ConTra tool. The regions were mapped onto the mouse genome (mm9) in the UCSC genome browser to determine their exact position in the promoter and first intronic region of the VEGFA gene.