FIGURE 5
FROM:
NG2, a novel proapoptotic receptor, opposes integrin 
4 to mediate anoikis through PKC-dependent suppression of FAK phosphorylation
N E Joo, T Watanabe, C Chen, M Chekenya, W B Stallcup and Y L Kapila
BACK TO ARTICLEFigure 5.
Overexpression of integrin 
4 rescues primary human fibroblasts from anoikis induced by an altered FN matrix. (a) Phase-contrast images of cells transfected with 4 siRNA (100 pmol), integrin 4 cDNA (1.5 
g), or transfection reagent. Cells were then treated with control serum-free medium (control) or with V+H+ or V+H-
protein (40 g/ml) for 4 h and photographed. Original magnification, 
200. (b, c) Flow cytometric analyses of apoptosis. Cells were transfected and treated as in (a). Western blots show the level of 4 expression after transfection. Actin served as a loading control. (d, e) Cell death detection ELISA assays of cells transfected and treated as in (a). Values are mean
S.E.M. *
P<0.05 versus control. **
P<0.05 versus V+H-
alone
