FIGURE 1
FROM:
Granzyme B-induced cell death exerted by ex vivo CTL: discriminating requirements for cell death and some of its signs
J Pardo, R Wallich, P Martin, C Urban, A Rongvaux, R A Flavell, A Müllbacher, C Borner and M M Simon
BACK TO ARTICLEFigure 1.
gzmB+CTLs induce membrane and mitochondrial perturbation in the absence of either Bid or Bak and Bax. Gp33-pulsed MEF.wt, MEF.Bid-/- (a, b) and MEF.Bak 
Bax-/- (c, d) cells were incubated with ex vivo virus-immune gzmB+CTL (MACS selected, 95% CD8+ cells) (1 h and/or 4 h, 10:1 effector/target ratio). Subsequently, PS exposure on plasma membrane (annexin-V-FITC) and PI uptake (a, c, e) and, in parallel, 
m loss (DiOC6(3)) and ROS generation (2-HE; b, d, f) were analyzed by three-color flow cytometry in the cell population negative for CD8 expression as described in Materials and Methods. A representative experiment is shown in the left panels. Numbers correspond to the percentage of cells in each quadrant. Data in the right panels are represented as the mean
S.E.M. of six (a, b), five (c) and four (d) independent experiments, respectively. (a, c) Histograms corresponding to the annexin-V staining in the dot plots are shown
